The comparative study of flavonoids in pear leaves of Noyabrska, Forest beauty and Lemonka varieties

Authors

DOI:

https://doi.org/10.24959/nphj.19.12

Keywords:

ordinary pear, flavonoids, qualitative and quantitative analysis, spectrophotometry

Abstract

Aim. To study the qualitative composition and determine the quantitative content of flavonoids in pear leaves of Noyabrska, Forest beauty and Lemonka varieties.
Materials and methods. As objects of research, leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties were selected. Identification of flavonoids was performed using qualitative reactions and thin-layer chromatography. The quantitative content was determined by the spectrophotometric method.
Results and discussion. The presence of flavonoids in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties was found by qualitative reactions. Using thin-layer chromatography the following components were identified in leaves of pears of the varieties studied: among glycosides – kosmosien, cynaroside, astragalin, rutin, hyperoside, quercitrin, isoquercitrin, among aglycons – apigenin, luteolin, kaempferol, quercetin. The content of flavonoids calculated with reference to rutin in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties was 2.88 ± 0.07%, 1.31 ± 0.03% and 1.64 ± 0.03%, respectively.
Conclusions. The study of the qualitative composition and quantitative content of flavonoids in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties has shown that they are not inferior to wild pear trees and can be an additional source of flavonoids for the purpose of creating herbal medicines on their basis.

Author Biographies

V. S. Kislychenko, National University of Pharmacy

Doctor of Pharmacy (Dr. habil.), professor, head of the Department of Chemistry of Natural Compounds

O. M. Novosel, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), associate professor of the Department of Chemistry of Natural Compounds

V. V. Korol, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), associate professor of the Department of Chemistry of Natural Compounds

References

Voitsekhivska, O. V., Sytar, O. V., Taran, N. Yu. (2015). Visnyk kharkivskoho natsionalnoho ahrarnoho universytetu. Seriia biolohiia, 1 (34), 104–119.

Khomych, H. P. (2013). Fenolni spoluky dykoroslykh plodiv i yahid: sklad, vlastyvosti, zminy pry pererobtsi: monohrafiia. Poltava : PUET, 217.

Fylymonenko, V. P., Zahaiko, A. L., Kochubei, Yu. I., Krasilnikova, O. A. (2017). Ukraina. Zdorov’ia natsii, 2 (43), 107–110.

Smirnov, O., Kosyk, O. (2011). Visnyk Lvivskoho universytetu. Seriia biolohichna, 56, 3–11.

Pinkevych, V. O., Novosel, O. M. (2018). Medychna ta klinichna khimiia, 20 (4). 136–140.

https://doi.org/10.11603/mcch.2410- 681X.2018.v0.i4.9826

Novosel, O. M. (2007). Farmakohnostychne vyvchennia predstavnykiv rodiv Malus ta Pyrus. Extended abstract of candidate’s thesis. Kharkiv : Raider, 20.

Katsuba, I. K., Kyslychenko, V. S., Novosel, O. M. (2011). Ukrainskyi medychnyi almanakh, 14 (6), 92–94.

Kyslychenko, V. S., Marchyshyn, S. M., Omelchenko, Z. I., Novosel, O. M., Khvorost, O. P., Zhuravel, I. O., Popyk, A. I. (2018). Metodyka pidhotovky ta provedennia laboratornykh zaniat z farmakohnozii: navch.-metod. posibnyk, 2. Ternopil: TDMU, 304.

Derzhavna farmakopeia Ukrainy. (2016). Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. (2–edition, dopovnennia 1). Kharkiv : Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”, 360.

Published

2019-08-21

Issue

Section

Synthesis and Analysis of Biologically Active Substances