http://nphj.nuph.edu.ua/issue/feedNews of Pharmacy2023-11-10T04:32:58+02:00Yelizaveta Volodymyrivna Zuikinanewspharm-journal@nuph.edu.uaOpen Journal SystemsPublication of original survey works on the main problems of pharmacy such as synthesis and analysis of biologically active substances, technology of medicines and control of their quality, organization and economy of pharmacy and pharmacoeconomics, preclhttp://nphj.nuph.edu.ua/article/view/286972The connection of the generations of Dmytro Pavlovych Salo technological school2023-09-04T16:57:00+03:00L. I. Vishnevskaliliiavyshnevska@gmail.comK. V. Semchenkotolochko.kv@gmail.comО. А. Rubanruban_elen@ukr.netN. P. Polovko polovko.np@gmail.comO. S. Kukhtenkotfp@nuph.edu.ua<p><strong>Aim</strong>. To study the development of the technological scientific school from the time of D. P. Salo to the present.</p> <p><strong>Materials and methods.</strong> Analytical, comparative and logical methods of research, as well as data from scientific literature, were used in the work.</p> <p><strong>Results and discussion.</strong> The development of the technological school through the prism of generations from D. P. Salo to the modern technological school of the National University of Pharmacy was studied.</p> <p><strong>Conclusions. </strong>The Department of Pharmaceutical Technology of Drugs of the National University of Pharmacy continues the good traditions of the technological school founded in the time of D. P. Salo, and actively introduces new modern trends in education and research.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/275361The study of the influence of pharmacotechnological and economic factors on the pharmaceutical development of capsules with trimetazidine for the treatment of cardiovascular diseases2023-03-13T14:46:37+02:00V. S. Zlahodazlagoda.vika@gmail.comL. O. Bobrytskalora2015dm@gmail.com<p>Cardiovascular diseases are the main cause of reduced life expectancy and quality in the modern world.</p> <p><strong>Aim. </strong>To study the influence of pharmacotechnological and economic factors on the pharmaceutical development of capsules with trimetazidine for the treatment of cardiovascular diseases.</p> <p><strong>Materials and methods.</strong> The research material was the medicinal substance trimetazidine (SharonBio-Medicine Ltd., India), excipients used in the technology of solid dosage forms.</p> <p>The study of pharmacotechnological characteristics was carried out on devices of the company “Pharma Test” (Germany). The fluidity and the angle of repose were measured on a VP-12A device. The fluidity was estimated by the Carr Index of powder compressibility and the Hausner Index coefficient. A comparative analysis of the cost calculation of the production of drugs with trimetazidine in tablet and capsulated forms was carried out using the “costs plus” method.</p> <p><strong>Results and discussion</strong>. As a result of the studies conducted on the selection of excipients for capsules with trimetazidine, the following excipients were reasonably used: lactose monohydrate 200/25 diluent, mannitol PARTECK M 200 filler and CompactCel®MAB mixture of a disintegrant, a moisture regulator and a lubricant.</p> <p>A comparative analysis of the cost calculation of the production of drugs with trimetazidine in tablets and capsules was carried out.</p> <p><strong>Conclusions.</strong> The advantages of direct encapsulation technology by economic and technological indicators prove the economic efficiency and contribute to new developments of high-quality medicinal products in capsules with high efficiency, availability and ease of use.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/286745Development of the technology for a transdermal therapeutic system of the anti-inflammatory action2023-08-31T20:46:40+03:00А. І. OlefirAnastasiy151994@ukr.netL. I. Vishnevskaliliiavyshnevska@gmail.com<p><strong>Aim. </strong>To develop the technology for manufacturing a transdermal therapeutic system of anti-inflammatory action in the laboratory and substantiate the choice of a support coating.</p> <p><strong>Materials and methods</strong>. For the study, model samples of the transdermal patch were used; their main components were a dry extract of white willow bark, a dry extract of sage leaves, quercetin, PVP, eudragit, PEO-400 and isopropyl alcohol – up to 100.0, as well as a support coating – a polyethylene terephthalate film. Organoleptic and technological research methods were applied.</p> <p><strong>Results and discussion</strong>. The rational technology of the laboratory production of an anti-inflammatory patch based on natural substances and the type of a support coating – a polyethylene terephthalate film with a thickness of 50 microns has been theoretically and experimentally substantiated. It has been found that the optimal mode of drying experimental patch samples is 50 °C, the drying time is 10 min. Keeping the samples in the thermostat for longer than 10 min, or at a temperature above 50 °C, leads to significant over-drying of the adhesive base, a film devoid of adhesive properties is formed, and it completely lags behind the polymer base. A flowchart for obtaining a patch has been developed, critical production parameters have been determined, and permissible deviation limits have been set.</p> <p><strong>Conclusions.</strong> The technology for manufacturing of an anti-inflammatory patch based on natural substances has been developed in the laboratory. The optimal support coating for the patch has been selected, the optimal drying mode of the adhesive composition has been determined. The critical parameters of the technological process for obtaining a patch have been determined.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/286613Research on the development of composition and technology of an emulgel with a probiotic component for the treatment of infectious and inflammatory diseases2023-08-30T16:35:45+03:00A. V. Soloviovasoloviova.alina@icloud.comO. S. Kalyuzhnayakalyuzhnayao.s@gmail.comN. V. Khokhlenkovahohnatal@gmail.comN. V. Dvinskykhbegunova1203@gmail.com<p><strong>The aim</strong> was conducted research to develop the composition and technology of a new soft preparation under the conventional name "Probioskin" for topical use with a probiotic component in the form of an emulgel.</p> <p><strong>Materials and methods of work.</strong> The homogeneity was determined by visual inspection of the experimental samples, using a biological microscope XSP-128 ULAB. The rheological (structural and mechanical) properties of the samples were studied using a Rheolab QC rheoviscosimeter (Anton Paar, Austria) with a C-CC27/SS coaxial cylinder system. The Rheolab QC rheometer is equipped with RheoPlus software. Microbiological studies and biotesting on a biological model of infusoria were performed under aseptic conditions of a laminar flow box (biological safety cabinet AC2-4E1 "Esco", Indonesia) of the Department of Biotechnology of the National University of Pharmacy. The identification of lactobacilli and the number of live cells as critical indicators of a preparation containing live microorganisms were performed at each stage of the study. Bacterioscopic control was carried out by examining smears prepared from samples of the dissolved drug and stained by Gram stain. Bacteriological control was performed by inoculation on thick MRS-4 medium and liquid MRS-1 medium. The number of live lactobacilli (when lactobacilli were co-cultured with the drug components at the stages of development and in the finished product) was determined by surface sowing on Petri dishes with thick MRS-4 medium; after 48 h of incubation at (37±1)°C, colonies grown on the surface of the medium were counted and the number of live lactobacilli expressed in CFU was calculated in 1 ml of the drug.</p> <p><strong>Results and discussion</strong>. Based on the conducted research, the composition of a soft preparation for topical use containing dexpanthenol, lactic acid, lyophilized lactobacillus biomass, propylene glycol, peach oil, polysorbate-80, aristoflex AVC, tocopherol, and purified water was developed. The production process of the developed Probioskin product is carried out according to a standardized scheme and consists 8 stages: preparation of raw materials, preparation of oil concentrate of the probiotic component, preparation of aqueous concentrate of active substances, preparation of gel base, preparation of emulsion, dosing into tubes, packaging of tubes into packs, packaging of packs into group containers.</p> <p><strong>Conclusions.</strong> On the basis of a complex research, the composition and rational technology of a soft preparation for topical use under the conventional name "Probioskin" in the form of an emulsion was developed.</p> <p><strong><em>Key words: </em></strong><em>pharmacological research, soft drug, emulgel, probiotics, dermatological diseases</em></p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/284135Development of the technology for the production of a medicinal product from fennel (Foeniculum vulgare) fruits in a filter bag2023-07-07T14:19:43+03:00O. M. Tkachuktkachuk.o.m@rma.edu.uaО. О. Shmalkoshmalko.a@gmail.comТ. М. Nesteruktnesteruk2020@gmail.comL. I. Vyshnevskaliliiavyshnevska@gmail.com<p><strong>Aim. </strong>To develop the technology of a medicinal product with a dry extract of fennel (<em>Foeniculum vulgare</em>) fruits in a filter bag with carminative, antispasmodic, analgesic, anti-inflammatory, antimicrobial and mild diuretic effects, which improves digestion, the motor evacuation function and the functional state of the gastrointestinal tract.</p> <p><strong>Materials and methods. </strong>To solve the tasks set in the work, such research methods as bibliosemantic, analytical and logical, generally accepted organoleptic (appearance, color, odor, etc.), biopharmaceutical (the study of the effect of the degree of grinding of the substance and excipients on the quality of the finished product), pharmacotechnological (description, weight of the contents of the container) and mathematical (statistical processing of results according to the requirements of the State Pharmacopoeia of Ukraine, using Statistica (StatSoft, USA) and Excel (Microsoft, USA) programs) were used.</p> <p><strong>Results and discussion. </strong>One of the biopharmaceutical indicators is a properly selected primary packaging, which will deliver the drug in a timely manner, ensuring the patient’s safety. Based on a complex of preliminary pharmacotechnological studies, the technology of the drug under the conditional name “Fenelmet, filter bags” has been developed.</p> <p><strong>Conclusions. </strong>Based on the research conducted, the technological process has been developed. The technology for the production of the medicinal product “Fenelmet, filter bags” and the draft technological regulations have been developed. Critical stages of the technological process have been identified; control parameters of critical stages and acceptance criteria that affect the quality indicators have been determined.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/287304Substantiation of the composition and technology of obtaining a dry extract of complex action in the laboratory conditions 2023-09-11T12:41:40+03:00О. О. Shmalkoshmalko.o@gmail.comV. K. Iakovenkov.iakovenko@gmail.com<p><strong>Aim. </strong>To substantiate the composition and extraction parameters of a multicomponent species for obtaining a dry extract of complex action.</p> <p><strong>Materials and methods.</strong> The study objects were the medicinal plant raw material, extractant and parameters of the extraction process. Information-search, information-analytical, organoleptic, physicochemical and pharmacotechnological research methods were used. The statistical processing of the data obtained was performed using Microsoft Excel 2007 spreadsheets.</p> <p><strong>Results and discussion</strong>. The study of the extraction conditions consisted in determining the optimal DER indicator by evaluating the nature of changes in the content of a dry residue, extractive substances in each separately collected sample of a liquid extract, as well as the concentration of groups of biologically active substances (BAS) in individual and total portions of extracts. The results obtained for the content of a dry residue and BAS show characteristic changes depending on the raw material : extract ratio. When the ratio increases from 1 : 1 to 1 : 15, there is a gradual decrease in the content of a dry residue in individual portions of the extract, the dynamics of the decrease in the concentration of individual groups of BAS (phenolic carboxylic acids, flavonoids, polysaccharides) is also unambiguous, but does not always correlate with changes in the content of extractive substances. Taking into account the results obtained, the extraction of the phytocomposition with 20 % ethanol at a DER ratio of 1 : 10 ensures an efficient and rational extraction in view of the content of BAS marker groups.</p> <p><strong>Conclusions.</strong> As a result of the work carried out, a dry extract has been obtained by the method of filtration extraction. The extraction process is carried out in laboratory conditions: the weight of the loaded raw material – 100.0 g; extractant – 20 % ethanol; extraction rate – 2-3 mL/min; the raw material : extractant ratio (DER) – 1:10. The yield of extractive substances is 26.68 %, the content of phenolic acids is 1.05 %, flavonoids – 0.25 %, polysaccharides – 1.01 %.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/286359The study of some quality parameters and the antioxidant activity of dietary supplements with the pomegranate extract (Punica granatum L.)2023-08-25T19:04:06+03:00O. Yu. Maslovalexmaslov392@gmail.comM. A. Komisarenko a0503012358@gmail.comS. V. Kolisnyks_kolesnik@nuph.edu.uaM. Yu. Golikaptekar4009@gmail.comS. R. Doroshenkosof.doroshenko@gmail.comO. V. Tkachenko kseniashovkova@gmail.comE. Yu. Akhmedovsuper.dan.96@ukr.netS. P. Karpova za9594506@gmail.com<p><strong>Aim. </strong>To conduct a qualitative, quantitative analysis and determine the antioxidant activity of dietary supplements with the pomegranate extract.</p> <p><strong>Materials and methods. </strong>Three dietary supplements from different manufacturers – “Extract of pomegranate” (manufactured by Source Naturals), “Extract of pomegranate” (manufactured by Puritan Pride), and “Extract of pomegranate” (manufactured by Vitacost) – were chosen for the study. The thin layer chromatography (TLC) was used to perform the qualitative analysis, the quantity of biologically active substances (BAS) in dietary supplements was determined by the spectrophotometric method, the antioxidant activity – by potentiometric method. The qualitative analysis was performed by thin-layer chromatography (TLC); spectrophotometry was used for the quantitative determination, and the potentiometric method was applied to determine the antioxidant activity.</p> <p><strong>Results and discussions. </strong>The presence of anthocyanins, flavonoids, catechins and phenolic acids were detected in the dietary supplements analyzed. The total content of phenolic compounds was 201.00 ± 6.03, 159.00 ± 4.77, 110.30 ± 3.30 mg, catechins was 16.10 ± 0.50, 11.60 ± 0.50, 7.33 ± 0.50 mg, anthocyanins was 43 ± 1.29, 32.00 ± 0.96, 22.00 ± 0.66 mg, and flavonoids was 30.15 ± 0.90, 29.44 ± 1.00, 18.21 ± 0.55 mg. The antioxidant activity was 266.11 ± 5.32, 212.12 ± 4.24, 150.81 ± 3.02 mmol-eqv./m<sub>extr</sub> for dietary supplements “Extract of pomegranate” (manufactured by Source Naturals), “Extract of pomegranate” (manufactured by Puritan Pride), “Extract of pomegranate” (manufactured by Vitacost) in 1 dosage form, respectively.</p> <p><strong>Conclusions. </strong>The analysis of the qualitative composition, the quantitative content of BAS and the antioxidant activity of dietary supplements with the pomegranate extract allows us to find out that dietary supplements manufactured by Source Naturals and Puritans Pride meet the requirements of the State Pharmacopoeia of Ukraine (SPhU), while a dietary supplement manufactured by Vitacost does not correspond the requirements. The results of the study allow us to state that the problem of compliance of dietary supplements is relevant; therefore, it is necessary to introduce normative documentation for the identification and determination of BAS in dietary supplements.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/272637The study of the state of awareness of pharmaceutical workers regarding coronavirus disease (COVID-19)2023-01-23T11:51:28+02:00O. M. Zaliskaolzaliska@ukr.netО. М. SemenovSsemenov.73@ukr.net<p><strong>Aim.</strong> To analyze and summarize the results of a questionnaire on the state of awareness of pharmaceutical workers in the Western regions of Ukraine about the coronavirus disease (COVID-19).</p> <p><strong>Materials and methods.</strong> The results of a survey of pharmacy managers, pharmacists and interns working in pharmacies in 8 regions of the Western area of Ukraine were used. The survey of respondents lasted 12 months: December 2021 – December 2022. A special questionnaire was developed to determine the level of knowledge about the coronavirus disease (COVID-19) caused by the SARS-CoV-2 coronavirus.</p> <p><strong>Results and discussion</strong>. With the beginning of the full-scale invasion of the Russian Federation, COVID-19 practically disappeared from the information space and ceased to be an urgent issue for Ukrainians. However, in recent weeks, the Ministry of Health has recorded an increase in the number of hospitalizations due to the coronavirus disease. During the period of martial law and the coronavirus disease pandemic (COVID-19), the pharmacy is the most accessible healthcare institution for the population and ranks first in terms of the number of citizens’ requests for symptoms, methods of treatment and prevention of SARS-CoV-2, therefore, the awareness of pharmacists – practitioners about COVID-19 is very important to provide complete and high-quality pharmaceutical care to patients. A review of the specialized scientific literature over the past 5 years confirmed the lack of studies on the level of awareness of pharmaceutical workers about the symptoms, methods of diagnosis, treatment and prevention of the main diseases that are widespread in the Western regions of Ukraine. With the help of a questionnaire, we conducted an expert assessment of the level of knowledge of pharmacy managers, pharmacists and interns working in pharmacy institutions in 8 regions of the Western area of Ukraine regarding the main symptoms, methods of diagnosis, treatment and prevention of uncomplicated forms of the coronavirus disease (COVID-19). After analyzing the answers of the respondents, it can be assumed that pharmaceutical workers know the main manifestations of the coronavirus disease (COVID-19) and are oriented towards the threatening indicators of the patient’s condition. It causes concern that some respondents incorrectly determine the reasons for prescribing antibacterial therapy to a patient, which can lead to incorrect recommendations and advice during the implementation of pharmaceutical care, however, the introduction of electronic prescriptions for dispensing antimicrobial drugs and information and educational work with pharmaceutical workers should correct the situation.</p> <p><strong>Conclusions.</strong> In our opinion, it is promising to conduct further research to improve the level of knowledge and competencies of pharmaceutical workers during their specialization courses and thematic improvement in the system of continuous professional development</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/281263The main aspects of effective internal audits of the energy management system in health care institutions2023-06-07T12:17:59+03:00M. P. Nosachenko19hagiri96@gmail.com S. M. Kovalenkosvetlana_kovalenko77@ukr.net<p><strong>Aim</strong>. To analyze the main components of conducting and forming recommendations for the effective implementation of internal audits of the energy management system in healthcare institutions.</p> <p><strong>Materials and methods</strong>. To achieve the goal, we applied such methods as informative, retrospective, analytical ones based on the study of scientific literature, materials of Internet-resources and our own conclusions regarding the results of the work carried out.</p> <p><strong>Results and discussion</strong>. The article analyzes the main aspects of effective implementation of internal audits of the energy management system in healthcare institutions. It has been shown that internal audits are not conducted effectively and do not add the expected value. The causes for low performance have been analyzed and systematized. It has been proven that the problem of improving the effectiveness of internal audits of the energy management system should be solved systematically, using the methods and approaches developed by the authors and proposed in the article.</p> <p><strong>Conclusions. </strong>Internal audits of the energy management system should be given closer attention in each healthcare institution since a properly organized audit process is an effective tool for analyzing the energy management system and provides important and relevant information for timely corrective and preventive actions.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/285174Marketing analysis and positioning of diuretic medicines at the pharmaceutical market of Ukraine2023-07-26T12:22:26+03:00V. V. Malyimalyi.vladimir@gmail.comN. G. Malininamalinina_nata@ukr.netO. Yu. Rohuliarogulyaolga@gmail.comG. S. Babichevababicheva.ann5@gmail.comI. V. Bondarievairyna.bondarieva@gmail.comA. А. Chehrynetskrutskih_ann@ukr.net<p><strong>Aim</strong>. To perform the marketing analysis of the domestic pharmaceutical market in the segment of diuretic medicines and study the directions of their positioning.</p> <p><strong>Materials and methods</strong>. The research materials are data from the State Register of Medicines of Ukraine and the Electronic Medicines Compendium. To achieve the goal, the methods of logical and meaningful problem formation, desk market research, comparative analysis, content analysis of scientific and practically oriented medical and pharmaceutical publications were used.</p> <p><strong>Results and discussion</strong>. The article presents the results of the marketing analysis of the domestic pharmaceutical market of diuretic medicines of group C03 “Diuretics” registered at the pharmaceutical market of Ukraine as of July 1, 2023. It has been determined that the range of diuretic medicines includes 124 trade names analyzed by INN, the number of trade names, by production, dosage form, price range, availability in the assortment of pharmacies. The analysis of the market of the group of medicines studied by producing countries, form of release and conditions of dispensing from pharmacies has been performed, as well as positioning has been carried by the peculiarities of the use of diuretics by such consumer segments as children, women during pregnancy and lactation. The INNs of medicines, which content are not offered by domestic manufacturers, have been identified, and therefore they can be considered promising for updating the product range.</p> <p><strong>Conclusions.</strong> The results of the marketing analysis of the diuretic market and development of positioning directions are the basis for the search and development of new effective domestic medicines for the treatment and prevention of renal failure, neurological and cardiac diseases and other diseases in the pharmacotherapy of which diuretics are used.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/285371Membrane-stabilizing properties of Viburnum opulus fruit liquid extract and cardioprotective effect of tablets based on it2023-07-30T21:33:48+03:00A. O. Yaroshenkoalina.yaroshenko1989@gmail.comO. Ya. Mishchenkomishchoksana@gmail.comO. S. Shpychakshpychak.oleg@gmail.com<p><strong>Aim.</strong> To study the membrane-stabilizing properties of <em>Viburnum opulus</em> fruit liquid extract (VOFLE) and the cardioprotective activity of “Viburnikor” tablets developed on the basis of VOFLE.</p> <p><strong>Materials and methods.</strong> Membrane-stabilizing properties of VOFLE were studied according to the Jager F.C. method in rats with determination of the spontaneous hemolysis degree caused by peroxide oxidation of erythrocyte membranes lipids. VOFLE (50, 100, 150 mg/kg), reference drugs Quercetin (200 mg/kg) and vitamin E (50 mg/kg) were administered to animals intragastrically (i/g) for 7 days. Adrenaline myocardiodystrophy was reproduced in rats by a single subcutaneous injection of adrenaline hydrochloride (0.18 % solution) in the dose of 0.5 mg/kg (0.28 ml/kg) of the body weight. Thirty (30) white male rats with an initial weight of 230-270 g, aged 6-6.5 months were used in the experiment. “Viburnikor” tablets (100 and 150 mg/kg of the VOFLE content) and Quercetin (200 mg/kg) were administered i/g for 7 days, the last time 1 hour before the cardiotoxin administration. The activity of the drugs was evaluated by the survival of animals, the functional state of the myocardium (ECG studies), the effect on the heart mass ratio, the activity of aspartate aminotransferase (AST), the level of active products interacting with thiobarbituric acid (TBA-AP), the content of reduced glutathione (RG) and catalase activity in the heart homogenate.</p> <p><strong>Results and discussion.</strong> The VOFLE studied showed the most pronounced membrane-stabilizing effect in the doses of 100 and 150 mg/kg, respectively, 22 and 34 %, the intensity of which was not inferior to the reference drugs Quercetin (26 %) and vitamin E (28 %). On the model of catecholamine myocardiodystrophy, “Viburnikor” tablets (100 and 150 mg/kg) contributed to the improvement of the heart function, as evidenced by the normalization of electrocardiographic parameters and heart mass ratio to the level of intact animals. It has been shown that the cardioprotective properties of “Viburnikor” tablets are exhibited by detecting anticytolic (decrease in the AST activity to the level of intact control) and antioxidant action (decrease in the level of TBA-AP in the myocardium of animals by 31 % (p<0.05) and 33 % (p<0.05), respectively, and a 1.6- and 1.7-fold increase in RG (p<0.05) and catalase (p<0.05) versus the control pathology).</p> <p><strong>Conclusions.</strong> It has been proven that the most pronounced membrane-stabilizing effect of the VOFLE studied is in the doses of 100 and 150 mg/kg. Cardioprotective properties of “Viburnikor” tablets have been determined on the model of catecholamine myocardiodystrophy by revealing anticytolic and antioxidant effects. By the intensity of the membrane-stabilizing and cardioprotective activity of the drugs studied, VOFLE and “Viburnikor” tablets are not inferior to the reference drugs.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/286681The IL-6-174C/G polymorphism analysis in Ukrainian residents as prospects for biomedical and pharmacogenetic uses2023-08-31T11:56:56+03:00O. V. Filiptsovaphiliptsova@yahoo.comO. I. Nabokaolganaboka201@gmail.comL. S. Petrovskal.s.petrovskaya96@gmail.comT. V. Martyniuktat.martynyuk@gmail.comS. G. Bobrosvetabobro1@gmail.comO. V. Tkachenkomedpharm@nuph.edu.ua<p>It is reliably determined that the presence of the <em>G</em> allele in the <em>174C/G</em> polymorphic region of the<em> IL-6 </em>gene promoter and a higher level of <em>IL-6</em> are more commonly observed among patients suffering from various metabolic disorders and obesity, malignant tumors, type 2 diabetes, periodontitis, oxidative stress. Over time, muscle damage and inflammation processes develop.</p> <p><strong>Aim.</strong> To study the frequency of the <em>IL-6-174C/G </em>single nucleotide polymorphism in the Ukrainian population.</p> <p><strong>Materials and methods. </strong>Buccal swab samples for the DNA analysis were collected from 102 healthy volunteers (48 males, 54 females, Ukrainian residents, predominantly ethnical Ukrainians). Genotyping to determine the <em>IL-6-174C/G</em> polymorphism was performed on DNA samples from the buccal epithelium using the polymerase chain reaction followed by RELP. Control of the genotype distribution for compliance with the Hardy–Weinberg equilibrium was performed using the χ<sup>2 </sup>criterion.</p> <p><strong>Results and discussion. </strong>The distribution of genotypes of the<em> IL-6-174C/G</em> polymorphism in the Ukrainian population samples was as follows:<em> CC</em> – 46 %, <em>CG</em> – 49 %, and <em>GG</em> – 5 % of residents. The frequency of the <em>IL-6-174C/G</em> polymorphism allele in the population was <em>p</em><em><sub>С </sub></em>– 0.71 and <em>q</em><em><sub>G </sub></em>– 0.29. The population structure did not deviate from the Hardy–Weinberg equilibrium since there was no difference between the theoretically expected and actual frequencies of three genotypes.</p> <p><strong>Conclusion. </strong>The data obtained demonstrates the presence of the <em>IL-6-174C/G </em>polymorphism in the Ukrainian population.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacyhttp://nphj.nuph.edu.ua/article/view/289959The study of the pharmacological activity of a cream with a white willow bark extract on the model of contact non-allergic dermatitis2023-10-26T17:59:47+03:00V. V. Pidgainavalentina.pidgaina@gmail.com<p>Despite the achievements of medicine, dermatological skin diseases are an urgent problem today as the incidence rate increases annually. A promising direction of modern pharmacotherapy of dermatoses is the creation of innovative etiotropic topical agents on a plant basis.</p> <p><strong>Aim. </strong>To study the pharmacological activity of a combined cream with a white willow (<em>Salix alba L.</em>) bark extract and zinc perithionate under the conditional name “Pyrisalix” against the background of the experimental non-allergic dermatitis induced by turpentine oil.</p> <p><strong>Materials and methods.</strong> As the study object, a cream made with the active substances of a thick extract of white willow bark and zinc perithionate on a cream base was used; it corresponded to the pharmacotechnological indicators by colloidal, thermal stability and the description of oil/water (20 % of the oil phase). The model of alternative chronic inflammation, non-allergic contact dermatitis, was induced in white non-linear rats by daily application of turpentine oil to the depilated area. The effectiveness of the “Pyrisalix” cream was evaluated based on the total leukocyte count, the peripheral blood leukocyte composition, erythrocyte sedimentation rate (ESR), including the leukocyte-ESR ratio index (LI-ESR), the skinfold thickness, and the skin cover condition. In our experiment, the phagocytic activity of granulocytic neutrophils in a leukocyte suspension obtained from heparinized blood was determined to assess the activity of nonspecific immune factors.</p> <p><strong>Results.</strong> The emulsion base of the cream has been determined, according to which the product developed has thermal and colloidal stability. In the course of studying the pharmacological activity of the “Pyrisalix” cream on themodel of non-allergic skin inflammation based on the dynamics of local manifestations of contact dermatitis, hematological, and immunological parameters, it has been found that the “Pyrisalix” cream has a pronounced preventive and therapeutic effect due to its anti-inflammatory and immunological properties. In the experimental conditions, the anti-alterative action of the cream on the emulsion oil/water base studied was equal to or even significantly exceeded the activity of the reference drugs – “Fladex” ointment and “Psorikap” cream by the intensity of inhibition of inflammatory processes in the skin, reduction in total leukocyte count, and normalization of the functional activity of granulocytic neutrophils.</p> <p><strong>Conclusions.</strong> The combined cream “Pyrisalix” on the emulsion oil/water base demonstrates a significant antiinflammatory activity on the model of non-allergic contact dermatitis. The combined cream “Pyrisalix” is a promising agent for further study in the treatment of dermatoses.</p>2023-10-23T00:00:00+03:00Copyright (c) 2023 National University of Pharmacy