Development of methods for analysis of the amount of flavonoids and their stability study in the combined dental gel
Keywords:dental gel, flavonoids, spectrophotometry, stability, pH
An important aspect in the pharmaceutical development of dental medicines is to provide them with a prolonged therapeutic effect while reducing the side effects of drug substances and the possibility of long-term use. This can be achieved by using active components of plant origin.
Aim. To develop methods for analyzing biologically active substances in the composition of a new combined dental gel.
Materials and methods. The study object was a dental gel containing “Phytodent” complex tincture (PJSC “CPP Chervona zirka”, Ukraine). Based on the analysis of the composition of the tincture it was proposed to carry out standardization by the amount of biologically active substances, namely flavonoids. Identification was carried out by TLC, while the quantitative determination by absorption spectrophotometry, the ultraviolet and visible method by the reaction with aluminum chloride using the standard method calculated with reference to rutin and the absorbance measurement at 406 nm.
Results and discussion. As a result of the research, the methods for the analysis of flavonoids in the composition of the new combined gel have been developed. The spectrophotometric method developed is characterized by specificity, accuracy, precision and linearity with r = 0.9998. One of the important issues when using components of plant origin is their stability both during preparation and storage. Using the method developed the stability of flavonoids has been studied depending on pH changes of the carbomer-based dental gel.
Conclusions. It has been determined that the methods developed are easily reproducible and allow to identifying and quantifying flavonoids in the dental gel. It has been found that a stable content of flavonoids is characteristic of the carbomer-based gel neutralized to pH values from 5.0 to 6.0.
Satyanarayana, S., Kumar, K. E., Rajashekhar, J., Thomas, L., Rajanna, S., Rajanna, B. (2007). Influence of aqueous extract of fenugreek seed powder on pharmacodyanamics and pharmacokinetics of gliclazide in rat and rabbits Therapy, 4, 457–463. doi: 10.1586/14750708.4.4.457.
Parasuraman, S., Thing, G. S., Dhanaraj, S. A. (2014). Polyherbal formulation: Concept of Ayurveda. Pharmacognosy Reviews., 8 (16), 73–80. DOI: 10.4103/0973-7847.134229.
Hasan, S. Z., Misra, V., Singh, S., Arora, G., Sharma, S. (2009). Current status of herbal drugs and their future perspectives. Biol Forum Int J., 1, 12–17. doi: 10.1038/npre.2007.1176.1.
Yuan, H., Ma, Q., Ye, L., Piao, G. (2016). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21 (5), 559. doi: https://doi.org/10.3390/molecules21050559.
Ishnava, K. B. (2018). Role of herbal medicine in dental health. J Environ Chem Toxicol., 2 (1), 28–29.
Petkovic, M. S., Kesic, L. G., Kitic, D. V., Milašin, J. M., Obradovic, R. R., Bojovic, M. D., Simonovic, A. A. (2015). Periodontal disease and phytotherapy. Org Hyg Health, 3, 1–4. doi: https://doi.org/10.4172/2332-0702.1000172.
Taheri, J. B., Azimi, S., Rafieian, N., Akhavan Zanjani, H. (2011). Herbs in dentistry. Int Dent J., 61, 287–296. doi: https://doi.org/10.1111/j.1875-595X.2011.00064.x.
Kumar, G., Jalaluddin, M., Rout, P., Mohanty, R., Dileep, C. L. (2013). Emerging trends of herbal care in dentistry. J Clin Diagn Res., 7, 1827–1829. doi: https://doi.org/10.7860/jcdr/2013/6339.3282.
Boddupalli, B. M., Mohammed, Z. N. K., Nath, R. A., Banji, D. (2010). Mucoadhesive drug delivery system: an overview. J. Adv. Pharm. Technol. Res., 1, 381–387. doi: https://doi.org/10.4103/0110-5558.76436.
Pragati, S., Ashok, S., Kuldeep, S. (2009). Recent advances in periodontal drug delivery systems. Int. J. Drug Deliv., 1, 1–14. doi: 10.5138/ijdd.2009.0975.0215.01001.
Kovalenko, S. M., Khokhlenkova, N. V., Azarenko, J. M. (2017). Methodological Aspects of Development of Gels for Treating Inflammatory Dental Diseases. Asian J. Pharm., 11, 129–135.
Phytodent®, tincture 100 ml. Available at: https://zvezda.kharkov.ua/en/catalog/fitodent-nastoyka-po-100-ml.html
Ruban, O. A., Maslii, Yu. S. (2019). Research on the choice of rational concentration of the gel forming agent in the composition of dental gel. Annals of Mechnikov Institute, 2, 29–33. doi: https://doi.org/10.5281/zenodo.3265076.
Maslii, Yu., Ruban, O., Levachkova, Yu., Gureyeva, S., Kolisnyk, T. (2020). Choice of Mucosal Adhesive in the Composition of a New Dental gel. Pharmakeftiki, 32 (1), 40–49.
Maslii, Yu. S., Ruban, O. A. (2018). The study of biopharmaceutical and adhesive characteristics of a dental gel. News of Pharmacy, 1, 28–32. doi: https://doi.org/10.24959/nphj.18.2188.
DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. (2015). Derzhavna Farmakopeia Ukrainy. (Vols. 1-3; Vol. 1). (2nd ed.). Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”, 1128.
European Directorate for the Quality of Medicines & Health Care. (2017). European Pharmacopoeia. (9th ed.). Strasbourg.
ICH harmonized tripartite guideline Q2 (R1). Validation of analytical procedures: text and methodology Q2 (R1). Proceedings of the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. Geneva, Switzerland, 2005.
Baliga, S., Muglikar, S., Kale, R. (2013). Salivary pH: A diagnostic biomarker. Journal ofIndian Society of Periodontology, 17 (4), 461–465. doi: https://doi.org/10.4103/0972-124X.118317.
Panzade, P., Puranik, P. K. (2010). Carbopol Polymers: A Versatile Polymer for Pharmaceutical Applications. Research Journal of Pharmacy and Technology, 3 (3), 672–675.
Kumara, P. T. P., Prakash N. K. S. C., Lokesh, P., Manral, K. (2015). Viscoelastic properties and rheological characterization of carbomers. International Journal of Latest Research in Engineering and Technology, 1 (6), 17–30. Available at: URL: http://www.ijlret.com/Papers/Vol-1-issue-6/4-A178.pdf.
Rabisková, M., Sedláková, M., Vitková, M., Kuna, M. (2004). Carbomers and their use in pharmaceutical technology. Ceská a Slovenská farmacie, 53 (6), 300–303.
Jurasekova, Z., Domingo, C., Garcia-Ramos, J. V., Sanchez-Cortes, S. (2014). Effect of pH on the chemical modification of quercetin and structurally related flavonoids characterized by optical (UV-visible and Raman) spectroscopy. Physical Chemistry Chemical Physics, 16 (25), 12802–12811. doi: https://doi.org/10.1039/C4CP00864B.
Pourcel, L., Routaboul, J. M., Cheynier, V., Lepiniec, L., Debeaujon, I. (2007). Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends in Plant Science, 12 (1), 29–36. doi: https://doi.org/10.1016/j.tplants.2006.11.006.
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