Development of methods for analysis of the amount of flavonoids and their stability study in the combined dental gel




dental gel, flavonoids, spectrophotometry, stability, pH


An important aspect in the pharmaceutical development of dental medicines is to provide them with a prolonged therapeutic effect while reducing the side effects of drug substances and the possibility of long-term use. This can be achieved by using active components of plant origin.

Aim. To develop methods for analyzing biologically active substances in the composition of a new combined dental gel.

Materials and methods. The study object was a dental gel containing “Phytodent” complex tincture (PJSC “CPP Chervona zirka”, Ukraine). Based on the analysis of the composition of the tincture it was proposed to carry out standardization by the amount of biologically active substances, namely flavonoids. Identification was carried out by TLC, while the quantitative determination by absorption spectrophotometry, the ultraviolet and visible method by the reaction with aluminum chloride using the standard method calculated with reference to rutin and the absorbance measurement at 406 nm.

Results and discussion. As a result of the research, the methods for the analysis of flavonoids in the composition of the new combined gel have been developed. The spectrophotometric method developed is characterized by specificity, accuracy, precision and linearity with r = 0.9998. One of the important issues when using components of plant origin is their stability both during preparation and storage. Using the method developed the stability of flavonoids has been studied depending on pH changes of the carbomer-based dental gel.

Conclusions. It has been determined that the methods developed are easily reproducible and allow to identifying and quantifying flavonoids in the dental gel. It has been found that a stable content of flavonoids is characteristic of the carbomer-based gel neutralized to pH values from 5.0 to 6.0.

Author Biographies

Yu. Maslii, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Pharmacy (Ph.D.), associate professor of the Department of Industrial Technology of Drugs

A. Materiienko, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Pharmacy (Ph.D), senior lecturer of the Department of Quality, Standardization and Certification of Drugs

O. Ruban, National University of Pharmacy of the Ministry of Health of Ukraine

Doctor of Pharmacy (Dr. habil.), professor, head of the Department of Industrial Technology of Drugs

I. Bezruk, National University of Pharmacy of the Ministryof Health of Ukraine

Ph.D. postgraduate student of the Department of Pharmaceutical Chemistry

L. Ivanauskas, Lithuanian University of Health Sciences.

Doctor of Biomedicine, professor, head of the Department of Analytical and Toxicological Chemistry


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Synthesis and Analysis of Biologically Active Substances