The biotechnological studies when developing lozenges with probiotics




probiotic, lozenges, ENT diseases


Aim. To develop lozenges with probiotic components for the treatment and prevention of ENT diseases and analyze their effectiveness.

Materials and methods. The study objects were lozenges based on xylitol and sorbitol of two compositions – based on live probiotic cultures and their metabolites. The following methods of control of the sample efficiency were
chosen: determination of the number of viable lactobacilli in the study of their combined use with the drug components and the number of viable bacteria in finished oral probiotics by the Koch method (direct seeding on cups), as well as the method of diffusion into agar with the growth inhibition zone determination.

Results and discussion. The studies of the properties of probiotic strains of L. fermentum 90-TC and L plantarum 8P-A3 have shown the prospects for their use in oral probiotics. It has been also proposed to use metabolites
of these strains as active components, which have a rather high antimicrobial potential to opportunistic pathogens that cause infectious ENT diseases. The oral probiotics in the form of lozenges of two compositions – based on live lactobacilli and their metabolites – have shown high efficiency, bacterial survival and antimicrobial properties, respectively.

Conclusions. During the complex of the theoretical, technological and biotechnological studies, lozenges with a probiotic of two compositions (based on viable probiotic cultures and their metabolites) have been developed. After further research they can be recommended as a biologically active additive to support the oral microflora, stimulate the own immunity and as a therapeutic and prophylactic agent in infectious diseases of the oral cavity for use in both adults and children.

Author Biographies

U. Starushenko, National University of Pharmacy of the Ministry of Health of Ukraine

student of the specialty of “Biotechnology and Bioengineering”

L. Yarova, National University of Pharmacy of the Ministry of Health of Ukraine

Master in “Biotechnology and Bioengineering”

O. Kaliuzhnaia, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Pharmacy (Ph.D.), associate professor of the Department of Biotechnology

N. Khokhlenkova, National University of Pharmacy of the Ministry of Health of Ukraine

Doctor of Pharmacy (Dr. habil.), professor, the head of the Department of Biotechnology

O. Kaliuzhnyi, Petro Vasylenko Kharkiv National Technical University of Agriculture

Candidate of Technical Sciences (Ph.D.), associate professor of the Department of Technology of Materials


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Technology of Medicines