The study of the diuretic activity and pharmacotechnological properties of a dry extract of Salvia sclarea L. growing in Tajikistan
DOI:
https://doi.org/10.24959/nphj.24.139Keywords:
clary sage (Salvia sclarea L.); dry extract; diuretic activity; pharmacotechnological properties; technology of solid dosage forms, hard gelatin capsulesAbstract
Aim. To study the pharmacological activity of a dry extract of clary sage (DECS) growing in Tajikistan and its pharmacotechnological properties as basic stages in the development of the composition and technology of hard gelatin capsules with its content.
Materials and methods. For the studies, we used DECS standardized by the content of the total amount of flavonoids and hydroxycinnamic acids. The quantitative content of the total amount of flavonoids was not less than 13.0 % calculated with reference to apigenin, and the quantitative content of the total amount of hydroxycinnamic acids was not less than 1.2 % calculated with reference to rosmarinic acid. The pharmacotechnological properties of DECS were studied according to methods of the State Pharmacopoeia of Ukraine (SPhU).
Results and discussion. During screening, all doses of DECS studied showed a moderate diuretic activity. Thus, when using DECS in the dose of 100 mg/kg, the relative volume of the urine excreted by rats for 5 hours increased by 1.9 times, in the dose of 200 mg/kg – by 2.0 times, in the dose of 300 mg/kg – by 1.8 times compared to the same indicator in the negative control group (p˂0.05). When studying the complex of pharmacotechnological properties, it was found that DECS was a finely dispersed amorphous powder with particles of isodiametric form. DECS had an average bulk weight of 0.515 ± 0.002 g/mL, and the fluidity assessment showed that DECS had a very poor flowability, which value in the vibration mode of the device was 2.8 ± 0.1 g/s. According to the study of the fractional composition, DECS had a clearly expressed fine fraction with a particle size of less than 0.25-0.09 mm 84.68 %.
Conclusions. Taking into account the results of the study of the diuretic activity and scientific literature data on the spectrum of the pharmacological activity of Salvia sclarea L, it is rational to consider DECS as a potential combined agent for enhancing the diuretic effect or in diseases of the urinary system accompanied by edema and inflammatory processes. The study of the pharmacotechnological indicators of DECS allows predicting the need to use certain groups of excipients to develop the composition and technology of capsules with its content, namely antifriction substances to improve fluidity and disintegrants to improve capsule disintegration, as well as substances that contribute to the compaction of bulk mass and moisture-regulating agents.
References
Hudz, N. I., Shanaida, M. I., Darmohrai, R. Ye. (2020). Shavliia likarska (Salvia officinalis L.): perspektyvy vykorystannia syrovyny yak dzherela roslynnykh preparativ antyoksydantnoi ta antymikrobnoi dii. Vistnyk farmatsi, 2, (100), 11-19.
Nizhenkovska, I. V., Tsurkan, O. O., Sedko, K. V. (2014). Shavliia likarska – suchasni aspekty zastosuvannia (Ohliad literatury). Fitoterapiia. Chasopys, 2, 58-61.
Koshovyi, O. M., Raal, A., Kovaleva, A. M., Myha, M. M., Ilina, T. V., Borodina, N. V. et al. (2020). The phytochemical and chemotaxonomic study of Salvia spp. growing in Ukraine. Journal of Applied Biology & Biotechnology, 8, (03), 29-36.
Myha, M. M., Koshovyi, O. M., Hamulia, O. V., Verkhovodova, Yu. V., Kireiev, I. V., Komisarenko A. M. (2020). Porivnialne farmakohnostychne ta farmakolohichne doslidzhennia lystia Salvia verticillata ta Salvia officinalis dlia vstanovlennia perspektyvy stvorennia novoho likarskoho zasobu. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 13, 1, 61-71.
Verkhovodova, Yu. V., Kireiev, I. V., Koshovyi, O. M., Myha, M. M. (2019). Doslidzhennia antyeksudatyvnoi aktyvnosti ekstraktiv z lystia Salvia officinalis. Medychna ta klinichna khimiia, 21, 4, 54-60.
Bones, M., Loizzo,M. R., Acquaviva, R., Malfa, G. A., Aiello, F., Tundis, R. (2017). Anti-inflammatory and Antioxidant Agents from Salvia Genus (Lamiaceae): An Assessment of the Current State of Knowledge. Anti–Inflammatory & Anti-Allergy Agents in Medicinal Chemistry, 16, (2), 70-86. doi: 10.2174/1871523016666170502121419.
Kostić, M., Kitić, D., Petrović, M. B., Jevtović-Stoimenov, T., Jović, M., Petrović, A. et al. (2017). Anti-inflammatory effect of the Salvia sclarea L. ethanolic extract on lipopolysaccharide–induced periodontitis in rats. Journal of Ethnopharmacology, 199, 52-59. doi: 10.1016/j.jep.2017.01.020.
Jasicka–Misiak, I., Poliwoda, A., Petecka, M., Buslovych, O., Shlyapnikov, V. A., Wieczorek, P. P. (2018). Antioxidant Phenolic Compounds in Salvia officinalis L. and Salvia sclarea L. Ecological Chemistry and Engineering S., 25, (1), 133-142. doi: 10.1515/eces-2018-0009.
Mervić, M., Bival Štefan, M., Kindl, M., Blažeković, B., Marijan, M., Vladimir-Knežević, S. (2022). Comparative Antioxidant, Anti–Acetylcholinesterase and Anti-α-Glucosidase Activities of Mediterranean Salvia Species. Plants, 11 (5), 625. doi: 10.3390/plants11050625.
Dudonne Xavier Vitrac, S., Philippe Coutière, Marion Woillez, Jean-Michel Mérillon (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J. Agric. Food Chem, 57, 1768-1774. doi: 10.1021/jf803011r.
Wu Zhi-Yu Ni, Y.-B., Qing-Wen Shi, Mei Dong, Hiromasa Kiyota, Yu-Cheng Gu, Bin Cong (2012). Constituents from Salvia Species and Their Biological Activities. Chemical Reviews, 112 (11), 5967-6026. doi: 10.1021/cr200058f
Firuzi, O., Miri, R., Asadollahi, M., Eslami, S., Reza Jassbi, A. (2013). Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iran. J. Pharm. Res, 12, 4. 801-810.
Durling, N. E., Catchpole O. J., Grey, J. B., Webby, R. F., Mitchell, K. A., Foo, L. Y. et al. (2007). Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures. Food Chemistry, 101 (4), 1417-1424. doi: 10.1016/j.foodchem.2006.03.050.
Verkhovodovoda, Y. V., Kireyev, I. V., Koshovyi, O. M., Osolodchenko, T. P. (2019). In vitro antimicrobial study of new modifications of salvia officinalis extracts. Annals of Mechnikov Institute, 1, 31-35. doi: 10.5281/zenodo.2639493.
Koshovyi, O. M., Perederii, Ye. O., Osolodchenko, T. P., Kovalova, A. M. Komisarenko, A. M. (2011). Doslidzhennia izoprenoidnoho skladu ta antymikrobnoi aktyvnosti hustoho ekstraktu lystia shavlii likarskoi. Klinichna farmatsiia, 15, 1, 26-29.
Dolia, V. S., Trzhetsynskyi, S. D., Mozul, V. Y., Tretiak N. Y. (2013). Osobennosty khymycheskoho sostava vydov roda Salvia L. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 3 (13), 83-85.
Sostav dlia lechenyia vospalytelnыkh zabolevanyi LOR orhanov : pat TJ 1063 Respublyka Tadzhykystan. № 1901381 ; zaiavl. 30.12.19 ; opubl. 17.02.20.
Musozoda, S. M., Rakhmonov, A. U., Makhsudov, K. S., Shpychak, O. S., Musoev, R. S., Kholova, Sh. S. et al. (2019). Anatomycheskoe stroenye assymyliatsyonnoho apparata shalfeia muskatnoho (Salvia scrlareae L.), proyzrastaiushcheho v Tadzhykystane. Nauka y ynnovatsyia, 4, 134-140.
Derzhavna farmakopeia Ukrainy : v 3 t. T. 1. (2015) / DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv». 2-e vyd. Kharkiv.
Kozhem’iakin, Yu. M., Khromov, O. S., Filonenko. M. A., Saifetdynova, H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (2010). Official Journal of the European Union, L 276, 33-79.
Doklinichni doslidzhennia likarskykh zasobiv : metod. rek. (2001). / za red. chlen-kor. AMN Ukrainy O. V. Stefanova. Kyiv.
Welu, G. G., Yimer, E. M., Hailu, H. G., Bhoumik, D., Lema, M. M. (2020). In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae). Evidence-based complementary and alternative medicine, 2020. doi: 10.1155/2020/1718708.
Meharie, B. G., Tunta, T. A. (2020). Evaluation of Diuretic Activity and Phytochemical Contents of Aqueous Extract of the Shoot Apex of Podocarpus falcactus. Journal of experimental pharmacology, 12, 629-641. doi: 10.2147/JEP.S287277.
Ayele, M., Makonnen, E., Ayele, A. G., Tolcha, Y. (2020). Evaluation of the Diuretic Activity of the Aqueous and 80 % Methanol Extracts of Ficus sur Forssk (Moraceae) Leaves in Saline-loaded Rats. Journal of experimental pharmacology, 12, 619-627. doi: 10.2147/JEP.S283571.
Indrayan, A., Malhotra, K. R. (2018). Medical biostatistics. 4th ed. Boca Raton.
Dopomizhni rechovyny v tekhnolohii likiv: vplyv na tekhnolohichni, spozhyvchi, ekonomichni kharakterystyky i terapevtychnu efektyvnist : navch. posib. dlia studentiv vyshch. farmats. navch. zakl. (2010). / avt.-uklad.: I. M. Pertsev ta in. ; za red. I. M. Pertseva. Kharkiv.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
