The effect of a new product based on quercetin and a thick extract of carrot seed root on the histological state of the rat cardiac muscle in the adrenaline-hydrocortisone-induced myocardial infarction
DOI:
https://doi.org/10.24959/nphj.24.150Keywords:
quercetin, thick carrot seed extract, experimental adrenaline-hydrocortisone myocardial infarction, cardioprotective effect.Abstract
Aim. To study the effect of tablets with a thick extract of carrot seed root and quercetin on the histological state of the rat cardiac muscle in the experimental adrenaline-hydrocortisone-induced myocardial infarction.
Materials and methods. The cardioprotective effect of tablets with a thick extract of carrot seed root and quercetin was studied on the model of acute adrenaline-hydrocortisone-induced myocardial infarction caused by subcutaneous administration of 0.1 % adrenaline hydrochloride solution in the dose of 1 mg/kg and 2.5 % hydrocortisone acetate emulsion in the dose of 12.5 mg/kg of the animal weight twice daily for a week. The tablets under research in the dose of 200 mg/kg and the reference drugs – “Thiotriazolin” and “Cratal” tablets were administered for 14 days – 7 days before the infarction modeling and 7 days of the myocardial infarction development, after which their effect on the histological state of the rat heart muscle was studied.
Results. Modeling of the adrenaline-hydrocortisone-induced myocardial infarction in rats is accompanied by the appearance of focal necrotic changes in muscle cells, which in some cases are accompanied by initial regenerative manifestations in the form of granuloma formation; dystrophic processes of cardiomyocytes. The therapeutic and prophylactic administration of tablets from a thick extract of carrot seed root and quercetin on the model of the adrenaline-hydrocortisone-induced myocardial infarction has the cardioprotective effect, reducing the severity of necrotic and dystrophic changes in the cardiac muscle.
Conclusions. As a result of modeling of the adrenaline-hydrocortisone-induced myocardial infarction in rats, focal necrotic changes in muscle cells were observed, sometimes accompanied by the initial formation of a matrix consisting of young fibroblasts; dystrophic processes of cardiomyocytes, in particular myocytolysis, fluctuations in the size of nuclei. The therapeutic and preventive administration of the tablets studied on the model of the adrenaline-hydrocortisone-induced myocardial infarction has the cardioprotective effect, reduces the severity of necrotic and dystrophic changes in the cardiac muscle. By the cardioprotective effect expressiveness, tablets with a thick extract of carrot seed root and quercetin on the model of the adrenaline-hydrocortisone-induced myocardial infarction are not inferior to the reference drugs – “Thiotriazolin” and “Cratal” tablets. The results experimentally substantiate the feasibility of creating a new combined drug from a thick extract of carrot seed root and quercetin for the prevention and pharmacocorrection of a cardiovascular pathology.
References
Movsisyan, N. K., Vinciguerra, M., Medina-Inojosa, J. R., Lopez-Jimenez, F. (2020). Cardiovascular Diseases in Central and Eastern Europe: A Call for More Surveillance and Evidence-Based Health Promotion. Annals of Global Health, 86(1), 21. doi: 10.5334/aogh.2713.
Townsend, N., Kazakiewicz, D., Wright, L. F., Timmis A., Huculeci, R., Torbica, A. et al. (2022). Epidemiology of cardiovascular disease in Europe. Nature Reviews Cardiology, 19(2), 133-143. doi: 10.1038/s41569-021-00607-3.
Murray, C. J. L. (2022). The Global Burden of Disease Study at 30 years. Nature Medicine. Nat Med, 28(10), 2019-2026. doi: 10.1038/s41591-022-01990-1.
Global Burden of Disease Collaborative Network.Global Burden of Disease Study 2019 (GBD 2019) Results. Seattle : Institute for Health Metrics and Evaluation (IHME), 2020. Available at : https://vizhub.healthdata.org/gbd-results/.
Global burden of 369 diseases and injuries in 204 countries and territories, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019. (2020). Lancet, 396(10258), 1204-1222. doi: 10.1016/S0140-6736(20)30925-9.
Kaptoge, S., Pennells, L., De Bacquer, D. (2019). World Health Organization cardiovascular disease risk charts: revised models to estimate risk in 21 global regions. Lancet Global Health, 7(10), 1332-1345.
Arvanitis, M., Lowenstein, C. J. (2023). Dyslipidemia. Annals of Internal Medicine,176(6), ITC81-ITC96. doi: 10.7326/AITC202306200.
Borghi, C., Fogacci, F., Agnoletti, D., Cicero, A. F. G. (2022). Hypertension and Dyslipidemia Combined Therapeutic Approaches. High Blood Press Cardiovasc Prev, 29(3), 221-230. doi: 10.1007/s40292-022-00507-8.
Kyslychenko, O. A., Paziuk, D.-M. V., Horiacha, L. M., Zhuravel, I. O. (2017). Identyfikatsiia fenolnykh spoluk morkvy posivnoi koreneplodiv ekstraktu hustoho. Relevant issues of modern medicine: the experience of Poland and Ukraine: International research and practice conference, (20-21 October 2017). (р.111-112). Lublin.
Kong, X., Liang, W., Li, X., Qiu, M., Xu, W., Chen, H. (2021). Characterization of an Acidic Polysaccharides from Carrot and Its Hepatoprotective Effect on Alcoholic Liver Injury in Mice. Chemistry & Biodiversity, 18(8), e2100359. DOI: 10.1002/cbdv.202100359.
Huwait, E. A., Saddeek, S. Y., Al-Massabi, R. F., Almowallad, S. J., Pushparaj, P. N., Kalamegam, G. (2021). Antiatherogenic Effects of Quercetin in the THP-1 macrophage model in vitro, with insights into its signaling mechanisms using in silico analysis. Frontiers in Pharmacology, 12, 698138. DOI: 10.3389/fphar.2021.698138.
Kononenko, T., Chikitkina, V. (2024). Eksperymentalne vyznachennia umovnoterapevtychnoi dozy novoho kombinovanoho hipolipidemichnoho zasobu na osnovi kvertsetynu ta morkvy posivnoi koreneplodiv ekstraktu hustoho na modeli hostroi hiperlipidemii. Fitoterapiia. Chasopys, 2, 137-146.
Shklianka, I. V., Zharinov, O. Y., Yepanchintseva, O. A., Todurov, B. M. (2019). Medykamentozna pidhotovka patsiientiv do khirurhichnoi revaskuliaryzatsii miokarda. Kardiokhirurhiia ta interventsiina kardiolohiia, 4, 5-14.
Zupanets, I. A., Holubovska, O. A., Shkurba, A. V., Shebeko, S. K., Shalamai, A. S. (2020). Perspektyvy vyvchennia zastosuvannia preparativ kvertsetynu v likuvanni COVID-19. Ukrainskyi Medychnyi Chasopys, 2(136), 75-78. doi: 10.32471/umj.1680-3051.136.177136.
Haman, D. V., Kononenko, N. M., Rybalkin, M. V. (2013). Patent Ukrainy 78443. Kharkiv.
Maynard, R., Downes, N., Finney, B. (2014). Histological techniques: an introduction for beginners in toxicology. Cambridge: Royal Society of Chemistry.
Kiernan, J. A. (2015). Histological and histochemical methods: theory and practice. 5th ed. Banbury: Scion Publishing.
Layton C., Bancroft J. D., Suvarna S. K. (2019). Fixation of tissues. Bancroft’s theory and practice of histological techniques 8 th ed. St. Louis: Elsevier.
Ullah, S., Ahmad, T., Ikram, M., Rasheed, H. M., Khan, M. I., Khan, T. et al. (2023). 7-Hydroxy Frullanolide Ameliorates Isoproterenol-Induced Myocardial Injury through Modification of iNOS and Nrf2 Genes. Biomedicines, 11(9), 2470. doi: 10.3390/biomedicines11092470.
Ganapathy, R., Ramachandran, A., Shivalingaiah, S. B., Bishir, M., Bhojaraj, S., Sridhar S. et.al. (2020). Cardioprotective potential of polyphenols rich Thraatchathi Chooranam against isoproterenol induced myocardial necrosis in experimental rats. BMC Complement Med Ther, 20(1), 356. doi: 10.1186/s12906-020-03124-x.
Hashmi, S., Al-Salam, S. (2015). Acute myocardial infarction and myocardial ischemia-reperfusion injury: a comparison. Int J Clin Exp Pathol, 8(8), 8786-8796.
Omar, A. M., Meleis, A. E., Arfa, S. A., Zahran, N. M., Mehanna, R. A. (2019). Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells Derived from Adipose Tissue and Bone Marrow on Acute Myocardial Infarction Model. Oman Med J., 34(6), 534-543. doi: 10.5001/omj.2019.97.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).