The study of the antimicrobial activity of a new anhydrous gel “Hypericum-derma” for the treatment of purulent wounds
DOI:
https://doi.org/10.24959/nphj.25.183Keywords:
purulent wound; gel; St. John’s wort; hawthorn; phenolic profile.Abstract
Aim. To determine the phytochemical composition and study the antimicrobial activity of a new anhydrous gel “Hypericum-Derm” for the treatment purulent wounds.
Materials and methods. The study objects were the anhydrous gel “Hypericum-Derm” containing α-arbutin, clotrimazole, lidocaine hydrochloride, the Hypericum perforatum herb extract, the Crataegus monogyna leaf and flower extract. The quantitative determination of the main biologically active compounds was performed by spectrophotometric and titrimetric method of analysis; the antimicrobial effect was assessed using the agar well diffusion method and the minimum inhibition concentration (MIC).
Results. The total content of polyphenols was 0.45 %, flavonoids – 0.35 %, organic acids – 0.16 %, hydroxycinnamic acid derivatives – 0.20 % and anthracene derivatives – 0.02 % in the anhydrous gel “Hypericum-Derm”. The experimental studies showed that the anhydrous gel “Hypericum-Derm” was active against strains of Pseudomonas aeruginosa (25.0 mm), Proteus vulgaris (25.0 mm), Escherichia coli (25.0 mm), Staphylococcus aureus (26.0 mm), Bacillus subtilis (25.0 mm) and Candida albicans (23.0 mm). The MIC values of the “Hypericum-Derm” gel was 0.018 mg/mL against S. aureus and B. subtillis, 0.035 mg/mL against P. aeruginosa, P. vulgaris, E. coli and C. albicans, which was 10 times lower than the MIC values of the reference drug “Levomekol”.
Conclusions. The phytochemical composition of the anhydrous gel “Hypericum-Derm” has been determined: phenolic compounds and flavonoids dominate in the gel, while anthracene derivatives have been found in the smallest amount. The anhydrous gel “Hypericum-Derm” has shown a high antimicrobial effect against Gram-positive and Gram-negative bacteria, and fungi. Thus, the anhydrous gel “Hypericum-Derm” can be used to treat purulent-necrotic wounds
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