The study of free coumarins in the plant raw material of medicago falcata l. Subsp. Romanica (prodan) O. Schwarz & klink
DOI:
https://doi.org/10.24959/nphj.14.1942Keywords:
Medicago falcata L. subsp. romanica (Prodan) O. Schwarz & Klink, pharmacognostic research, coumarinsAbstract
The article presents the data of the pharmacognostic research of the plant raw material from the overground parts of Medicago falcata L. subsp. romanica (Prodan) O. Schwarz & Klink. The aim of our work was to determine the composition and the quantitative content of the components from the true coumarins class revealing the anticoagulation effect. The plant raw material was collected in the period of active vegetation. It was dried in a draught at the temperatures up to 40°C. For the first time gas-liquid chromatography with mass spectrometric detection of the raw material extract has been performed. The components have been identified by the library of mass spectra NIST 05 and WILEY 2007 together with the programmes for identifying AMDIS and NIST. The internal standard method has been used for quantitative calculations. The data obtained have shown the presence of 66 compounds, and 38 of them have been identified and quantitatively characterized. The extract from the raw material of Medicago falcata L. subsp. romanica (Prodan) O.Schwarz & Klink contains a number of biologically active substances in the native form – fatty acid and primary products of biosynthesis. Dihydrocoumarin and coumarin in the corresponding amounts have been identified from compounds of the true coumarin class in the overground parts of alfalfa (M. falcata L. subsp. romanica).
References
Дідух Я.П., Бурда Р.І., Зиман С.М. та ін. Екофлора України. – К.: Фітосоціоцентр, 2004. – Т. 2. – 480 с.
Канівець В.І., Канівець С.В. // Грунтознавство. – 2008. – Т. 9, №1-2. – С. 48-52.
Новоселова Л.В., Росков Ю.Р., Качкина А.Б. // Вестник Пермского университета. Серия Биол. – 2004. – Вып. 2. – С. 64-71.
Шапаренко І. // Вісник Львівського університету. Серія Біол. – 2012. – Вип. 58. – С. 97-106.
Cieśla Ł., KowalskaI., Oleszek W. еt al. // Phytochemical Analysis. – 2013. – Vol. 24, №1. – P. 47-52.
Ebrahimzadeh M.A., Pourmorad F., Bekhradnia A.R. // African J. Biotechnol. – 2008. – Vol. 7, №18. – P. 3188-3192.
Kancheva V.D., Boranova P.V., Nechev J.T. еt al. // Biochimie. – 2010. – Vol. 92. – P. 1138-1146.
Kowalska I., Stochmal A., Kapusta I. et al. // J. Agric. Food Chem. – 2007. – Vol. 55. – P. 2645-2652.
Mirzaei1 A., Abbasi M., Sepehri S. et al. // Life Sci. J. – 2013. – Vol. 10, №11. – P. 27-31.
Olech M., Komsta Ł., Nowak R. et al. // Food Chem. – 2012. – Vol. 132. – P. 549-553.
Stochmal A., Kowalska I., Janda B. et al. // Phytochemistry. – 2009. – Vol. 70. – P. 1272-1276.
Downloads
Published
Issue
Section
License
Copyright (c) 2014 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
