Investigation of the composition of active pharmaceutical ingredients in “Apised” capsules by the method of high performance liquid chromatography
Keywords:terpenoids, flavonoids, essential oils, high-performance liquid chromatography
As high-performance liquid chromatography is the most optimal method for qualitative and quantitative analysis of multicomponent medicines, including phytomedicines, – owing to the possibility to identify and quantify the substances of different volatility degree in the same sample, – the qualitative composition and quantitative content of the complex apiphytomedicine “Apised” developed in the form of capsules for treatment of mental and emotional disorders and depression states in sports medicine has been investigated using HPLC-analyser on the basis of a Milichrome A-02 chromatograph. At the preliminary stage of our research of the active pharmaceutical ingredients of “Apised” capsules and the apiphytomedicine directly developed it has been found that there are 14 compounds in the composition of the standardized substance of natural powdered honey, 19 compounds in the composition of the garden balm herb, 24 compounds in the composition of the hop cones, 20 compounds in the composition of the inflorescences of spike lavender, 27 compounds in the composition of “Apised” capsules. By comparing the retention times and spectral ratios of the peaks obtained with the device database the presence of linalool, rosmarinic and chlorogenic acids in the composition of the garden balm herb, quercetin and myrcene in the composition of the hop cones, linalool in the composition of the inflorescences of spike lavender, linalool and myrcene in the composition of natural powdered honey has been shown. The experimental data concerning the percentage of biologically active substances in the apiphytomedicine “Apised” correlate well with the composition of the medicine developed and the literary information about the chemical composition of the medicinal plant raw material studied. The method offered gives the possibility to carry out standardization of the composition of the medicine developed, namely “Apised” capsules, regardless of the way of obtaining and origin of the medicinal plant raw material.
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