The antimicrobial activity of silver nanoparticles in vitro
DOI:
https://doi.org/10.24959/nphj.15.2002Keywords:
silver nanoparticles, antimicrobial activity, spectrum of bacteriaAbstract
Silver nanoparticles possess a high potential as an antimicrobial substance against a wide spectrum of bacteria, including antibiotic-resistant strains. Antimicrobial properties of silver nanoparticles with 30 nm in diameter synthesized according to the original protocol have been determined in this study. In in vitro study using the serial dilutions method in the solid medium the minimal inhibition concentration (MIC) of silver nanoparticles against such test-strains as Staphylococcus aureus MRSA ATCC 43300, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 2592, Shigella sonnei, Salmonella typhimurium 144 was equal to 33.46 μg/ml. MIC against B. subtilis ATCC 6633 was 133.8 μg/ml. The antimicrobial activity of silver nanoparticles has been studied on clinical isolates with multiple drug resistance isolated from wounds, urine, endocervical and faucial scrapings in surgical patients with Klebsiella ozaenae 4348, Citrobacter freundii 4369, Escherichia coli 4358, Enterobacter aerogenes 2476, Proteus mirabilis 4363, Staphylococcus aureus 4312 and Pseudomonas aeruginosa 283. The total inhibition of the microorganisms growth under the action of both doses of silver nanoparticles studied – 10 μg and 20 μg has been observed.References
Грузіна Т.Г., Дибкова С.М., Прискока А.О та ін. // Фармакологія та лікарська токсикологія. – 2012. – №3. – С. 40 – 46.
Методические указания МУК 4.2.1890-04 // Клин. микробиол. антимикроб. химиотер. – 2004. – Т.6, №4. – С. 306-359.
Патон Б.Є., МоскаленкоВ.Ф., Чекман І.С., Мовчан Б.О. // Вісник Національної академії наук України. – 2009. – № 6. – С. 76–80.
Чекман І.С. Нанофармакологія. – К.: Задруга, 2011. – 424 с.
Чекман І. С., Симонов П.В. Природні наноструктури та нано-механізми. - К.: Задруга, 2012. – 104 с.
Чекман І.С., Ульберг З.Р., Маланчук В.О. та ін. Нанонаука, нанобіологія, нанофармація. – К.: Поліграф плюс, 2012. – 328 с.
Banerjee M., Mallick S., Paul A. et al. // Langmuir. – 2010. – Vol. 26, №8. – P. 5901 – 5908.
Bhattacharaya R., Mukherjee P. //Advanced drug delivery reviews. – 2008. – Vol. 60. – P. 1289 – 1306.
Bhol K.C., Alroy J., Schechter P. J. //Clin. Exp. Dermatol. – 2004. – Vol. 26, №3. – P. 282 – 287.
Das V.L., Tomas R., Varghese R.T. et al // 3 Biotech. – 2014. – Vol. 4. – P. 121 – 126.
Dunn K.// Burns. – 2004. – Vol. 30. – P. 1 – 9.
Gunawan C., Teoh W.Y., Marquis C.P. et al. // Small. – 2013. – Vol. 9, №21. – P. 3554–3560.
Hong H.A., Khaneja R.; Tam N. et al. // Research in Microbiology. – 2009. – Vol. 160, № 2. – P. 134 – 143.
Jain J., Arora S., Rajwade J. M. et al. // Mol. Pharm. – 2009. – Vol. 6, №5. – P. 1388 – 1401.
Kahru A., Dubourguier H.-C. // Toxicology. – 2010. – Vol. 269. – P. 105 – 119.
Kalimuthu K., Babu R.S., Venkataraman D. et al. // Colloids and Surfaces B: Biointerfaces. – 2008. – Vol. 65. – P. 150 – 153.
Li Q., Mahendra D., Lyon D. et al. // Water research. – 2008. – Vol. 42. – P. 4591 – 4602.
Nagy A., Harrison A., Sabbani S. et al. // Int. J. Nanomedicine. – 2011. – Vol. 6. – P. 1833 – 1852.
Pal. S., Tak K. Y., Song J. M. // Applied and environmental Microbiology. – 2007. – Vol. 73, № 6. – P. 1712 – 1720.
Roe D., Karandikar B., Bonn-Savage N. et al. // Journal of antimicrobial chemotherapy. – 2008. – Vol. 61. – P. 869 – 876.
Zhang W., Li Y., Niu J., Chen Y. // Langmuir. – 2013. – Vol. 29, №15. – P. 4647 – 4651.
Downloads
Published
Issue
Section
License
Copyright (c) 2015 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).