The chromatographic study of the extracts of glibenclamide, gliclazide and glimepiride obtained from biological objects


  • T. V. Kucher National University of Pharmacy, Ukraine
  • S. I. Merzlikin National University of Pharmacy, Ukraine



drugs, sulphonylurea derivatives, chemico-toxicological analysis, biological objects, methods of isolation, TLC method


This article presents the results of the standardized conditions elaboration for detection and identification of sulfonylureas derivatives (SUD) such as glibenclamide, gliclazide and glimepiride obtained by chromatographing their extracts from the liver tissues using TLC method. The isolation of SUD from a biological object has been conducted with the help of general methods for chemico-toxicological analysis of drugs such as extraction with water acidified by oxalic acid according to Vasileva method and extraction with ethanol acidified by oxalic acid according to Stas-Otto method. The chloroform extracts obtained have been examined according to the methodology of TLC-screening of drugs in two stages. At the first stage ethyl acetate as the common mobile phase was used, and at the second stage – the special systems for the substances studied such as ethyl acetate – glacial acetic acid (49.5:0.5) and methylene chloride – ethyl acetate – glacial acetic acid (50:50:1). For detection of their adsorption zones the specific reagents were used: 1% solution of vanillin and 5% solution of chloral hydrate. It has been found that the chloroform extracts of SUD previously introduced in the model sample of the liver in the concentration of 20 mg per 50 g of the biological object and isolated from this sample by Stas-Otto method have satisfactory Rf values that meet the chromatographic parameters of Rf values of the standard substances. However, when chromatographing the chloroform extracts of SUD introduced in the biological object in the same concentration and isolated from it by Vasileva method the absorption zones in the thin layer have not been found. With increase in concentrations of the compounds studied to 50 mg per 50 g of the biological object and subsequent chromatography of the corresponding chloroform extracts obtained by Vasileva method, their Rf value did not correspond to the chromatographic parameters of the standard substances. Thus, the application of Vasileva method to obtain the extracts of the SUD under study from the liver tissues has a negative impact on their detection sensitivity and parameters of the chromatographic mobility in the thin layer of the sorbent. It has been determined that the proposed and standardized conditions for detection and identification of drugs from SUD by TLC are suitable for chemico-toxicological analysis of chloroform extracts obtained from the liver tissues by Stas-Otto method.

Author Biographies

T. V. Kucher, National University of Pharmacy

Department of the Toxicological Chemistry

S. I. Merzlikin, National University of Pharmacy

Professor of the Toxicological Chemistry Department


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