The qualitative and quantitative analysis of volatile compounds of the herb of dahlia nymphaeales of Ken’s Flame cultivar


  • N. I. Ilyinska National University of Pharmacy, Ukraine
  • T. M. Gontova National University of Pharmacy, Ukraine
  • Ya. S. Krukova National University of Pharmacy, Ukraine



sesquiterpenoids, fatty acids, sesquiterpene ethers/essential oils, Dahlia, herbs


Many plants consist of volatile substances, these substances possess a variety of pharmacological effects on the human body, the main ones are analgesic, anti-inflammatory, healing, antimicrobial, soothing, and so on. The aim was to study the qualitative composition and quantitative content of volatile substances of the herb of Dahlia Nymphaeales of Ken's Flame cultivar by chromatography-mass spectrometry. For the study an Agilent Technology 6890 gas chromatograph with the mass spectrometric detector 5973 was used. As the result of the research 46 substances have been determined, among them 43 substances have been identified. It has been found that sesquiterpinoids – α-kadinol, globulol, vulgarol A, spathulenol, allo-spathulenol were dominant among all groups of compounds identified. The quantitative content of this group was 21.9% of the total amount of compounds. The content of both sesquiterpenoids and fatty acids was the same (17.7% each). Sesquiterpinoids were represented by 10 compounds: aromadendren, allo-aromadendren, β-Elemen, β-farnezen, germacrene D, germacrene B, shyobunon, farnezen, δ-kadinen, β-sesquiphellandrene. Shiobunon prevailed among sesquiterpinoids (210.81 mg/g). The fatty acids were represented by 4 compounds: palmitoleic acid, palmitic acid, oleic acid, linoleic acid, among them palmitoleic acid (200.46 mkg/g) prevailed. The content of triterpenoids (β-caryophyllene, α-caryophyllene, squalene) was 9.11%. In addition to these compounds the essential oil of Dahlia Nymphaeales herb contained alkanes (8.7%), sesquiterpene ketones (7.9%), diterpene alcohols (5%). Furthermore, the raw material accumulated a trace amount of alkenylphenols (1.6%), ketones (1.4%), sesquiterpene oxides, aldehydes and phenols (1.2% each), monoterpene alcohols (0.32%).


Author Biographies

N. I. Ilyinska, National University of Pharmacy

Postgraduate student

T. M. Gontova, National University of Pharmacy

Head of Botany Department

Ya. S. Krukova, National University of Pharmacy

Associate Professor of Botany Department


Гонтова Т. М., Кічимасова Я. С., Ільїнська Н. І. // Збір. наук. праць співробіт. НМАПО ім. П. Л. Шупика 2014. – Вип. 23, (4). – С. 245-249.

Плахотнича Є. А., Ільїнська Н. І., Кічимасова Я. С. // Досягнення сучасної медицини: матеріали 75-ї загальноуніверситетської студ. наук. конф., м. Львів 24-25 квіт. 2014 р. – Л., 2014. – С. 222–223.

Astani A., Reichling J., Schnitzler P. // Phytotherapy Research. – 2010. – Vol. 24 (5). – Р. 673-679.

Bassole I., H. R. Juliani // Molecules. – 2012. – Vol. 17 (4). – P. 3989-4006.

Bicchi C., Brunelli C., Cordero C.// J. Chromatogr. A. – 2004. – Vol. 1024, № 1-2. – P. 195-207.

Ilyinska N., Т. Gontova, Y. Kichymasova // Actual Questions Of Development оf New Drugs : XX International scientific аnd рractical сonference оf young scientists and student, аpril 22-23, 2014, Kharkiv. – Р. 32.

Khan M. A. Chervinskaya A. V., Sotnikova E. N. // Problems of balneology, physiotherapy and exercise therapy. – 2005. – Vol. 2. – P. 9-12.

Kovaleva A. M., Goncharov N. F., Komissarenko A. N. et al. // Chemistry of Natural Compauds. – 2009. – Vol. 45, № 4. – P. 592-593.

Roman M., Dobrowolski J. C., Baranska M. et al. // Journal of natural products. – 2011. – Vol. 74 (8). – P. 1757-1763.

Sacchetti G., Maietti S., Muzzoli M. et al. // Food chemistry. – 2005. – Vol. 91 (4). – Р. 621-632.

Sokolova O., Gontova T., Ilyinska N., Matkowski A. // Trends in natural products research: young scientists meeting, June 23-25, 2014, Olomouc. – P. 58-59.

Weitzel C. // Phytochemistry. – 2011. – Vol. 72. – P. 572-578.






Synthesis and Analysis of Biologically Active Substances