Optimization of the detection conditions for the series of 1,2,4-triazole-3-thiones for fia-esi-ms
Keywords:flow injection analysis (FIA), high performance liquid chromatography (HPLC), mass-spectrometry (MS), electrospray ionization (ESI), 1, 2, 4-triazole-3-thions, optimization, polynomial regression
Development of methods for determination of 1,2,4-triazole-3-thiones as intermediate substances in the synthesis of 1,2,4-triazole-3-thioacetate acids salts as potential drugs is an important task at the research and production stage. The study of absorption, distribution, metabolism and excretion requires development of the corresponding methods. The most universal and selective methods used in these cases is the HPLC-MS.The aim of this study was to optimize the conditions of mass spectrometric detection for FIA-MS (flow injection analysis with mass-spectrometric detection) and HPLC-MS (high performance liquid chromatography with mass-spectrometric detection) with the electrospray ionization of eight 1,2,4-triazole-3-thiones by three factors of full factorial design and polynomial regression equations. The work has been performed using the technique of direct sample introduction into the ion source (flow injection analysis - FIA) on an Agilent 1260 Infinity HPLC system with an Agilent 6120 single quadrupole mass spectrometer. The equations of polynomial regression dependence of the signal intensity of the mass detector on three important factors such as the gas drier temperature, the fragmentor voltage and the nebulizer gas pressure have been calculated for the substances studied. Based on the location of the maxima of the functions obtained the optimal values of these factors have been determined. The choice of optimal conditions of mass spectrometric detection allows to maximize the signal on the detector and thus to increase the sensitivity and selectivity of determinations.
Каплаушенко А.Г. Синтез, будова і біологічна активність похідних 4-моно- та 4,5-дизаміщених 1,2,4-триазол-3-тіону: Автореф. дис. ... докт. фарм. наук.: 15.00.02. / Запорізький держ. мед. ун-тет, 2012. – 43 с.
Каплаушенко А.Г., Панасенко О.І., Книш Є.Г. та ін. // Фармац. журн. – 2008. – №2. – C. 67-72.
Самелюк Ю.Г., Каплаушенко А.Г. // Актуальні питання фармац. та мед. науки та практики. – 2013. – №2. – С. 125-128.
Щербіна Р.О., Панасенко О.І., Книш Є.Г. та ін. // Актуальні питання фармац. та мед. науки та практики. – 2014. – №3. – С. 18-21.
Cremin P., Kasim-Karakas S., Waterhouse A.L. // J. Agric. Food Chem. – 2001. – Vol. 49. – P. 1747-1750.
Gratz S.R., Flurer Ch.L., Wolnik K.A. // J. Pharmac. Biomed. – 2004. – Vol. 36. – P. 525-533.
Haverford college. Department of Chemistry. Resources. Instruments. Analysis by Direct Injection on the Agilent LC/MS. http://www.haverford.edu/chem/Agilent%20Direct%20Inject.pdf
Huang L.-F. et al. // J. Pharmac. Biomed. – 2003. – Vol. 33. – P. 1155-1162.
Lin L.-Z. et al. // J. Agric. Food Chem. – 2000. – Vol. 48. – P. 354-365.
Takino M. Determination of the metabolites of nitrofuran antibacterial drugs in chicken tissue by LC-ESI-MS, Agilent Technologies, 2003 (application note).
Copyright (c) 2016 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).