The amino acid and monosaccharide composition of a dry extract from Salvia officinalis leaves obtained by complex processing

Authors

  • G. V. Vovk National University of Pharmacy, Ukraine
  • O. M. Koshovyi National University of Pharmacy, Ukraine
  • A. M. Komissarenko National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/nphj.16.2071

Keywords:

Salvia officinalis, leaves, dry extract, amino acid, monosaccharide, complex processing

Abstract

The amino acid and monosaccharide composition of a dry extract from Salvia officinalis leaves obtained by complex processing has been studied. The preliminary determination of amino acids and monosaccharides has been performed by paper chromatography. The qualitative and quantitative analysis of free and bound amino acids and monosaccharides in the extract from Salvia officinalis leaves has been carried out using a high performance liquid chromatograph by Agilent Technologies company (model 1100) equipped with a flow vacuum degasser G1379A, a 4-channel pump of the low pressure gradient G13111A, an automatic injector G1313A, a column thermostat G13116A, diode array detector G1316A. As a result of the chromatographic studies of the amino acid and monosaccharide composition in the dry extract from Salvia officinalis leaves 10 free and 12 bound amino acids have been identified, six of which are indispensable – threonine, valine, isoleucine, leucine, phenylalanine and arginine, and 4 monosaccharides – glucose, galactose, rhamnose and arabinose. In the extract of Salvia officinalis leaves dominant substances are tyrosine, serine, glutamic and aspartic acid. In the dry extract of Salvia officinalis leaves the content of free (0.38%) and bound (0.43%) amino acids, monosaccharides (6.9%) increasing up to 11.2% after hydrolysis has been determined.

Author Biographies

G. V. Vovk, National University of Pharmacy

the post-graduate student of Pharmacognosy Department

O. M. Koshovyi, National University of Pharmacy

Head of Pharmacognosy Department

A. M. Komissarenko, National University of Pharmacy

Professor of Chemistry of natural compounds Department

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Published

2016-03-11

Issue

No. 1(85) (2016)

Section

Synthesis and Analysis of Biologically Active Substances

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