The effects of goutweed (Aegopodium podagraria L.) preparations on glycemia in intact rats and against the background of metformin
DOI:
https://doi.org/10.24959/nphj.17.2161Keywords:
Aegopodium podagraria L., glycemia, metformin, ratsAbstract
The preparations obtained from goutweed (Aegopodium podagraria L.) are characterized by favorable metabolic effects, including the antidiabetic activity.
Aim. To determine the dose dependence of the effect of the extract and the tincture of goutweed on the glucose metabolism in intact rats, to evaluate the metabolic effects and the possibility of the combined use of the goutweed preparations with metformin.
Materials and methods. The experiments were conducted on intact rats. The influence of the extract and the tincture of goutweed (per se and combined with metformin) on the basal glycemia and the results of the glucose tolerance test were determined. The metabolic effects which are possibly interrelated (the changes in uric acid and electrolyte exchange) were also studied.
Results and discussion. The goutweed tincture exhibits the hypoglycemic action in intact rats after administration of single doses of 0.5; 1.0; 5.0 ml/kg, while the goutweed extract (100; 250; 500 mg/kg and 1.0 g/kg) does not demonstrate this action. The correlation analysis results indicate that certain interrelationship may exist between the effect of the extract on the electrolyte homeostasis and glucose metabolism (though these changes are moderate in intact animals), as well as between the effect of goutweed preparations on uric acid and the glucose exchange. The goutweed tincture in the doses of 0.5 and 1.0 ml/kg does not block the effects of metformin, and does not enhance its effect with the excessive decrease of the blood glucose concentration. The absence of a significant decrease of glycemia after combined administration of the tincture and low doses of metformin may reflect synergoantagonism (however, these phenomena were seen in intact animals, and the dose dependence of the metformin action did not change). In the glucose tolerance test the tincture in the dose of 1.0 ml/kg (but not 0.5 ml/kg) combined with metformin allows reducing its effective dose from 400 mg/kg to 200 mg/kg.
Conclusions. Goutweed tincture, in contrast to the extract, shows a dose-dependent hypoglycemic effect and is promising for combined use with metformin.
References
Rehman, S. U., Choi, M. S., Choe, K., Yoo, H. H. (2014). Interactions between herbs and antidiabetics: an overview of the mechanisms, evidence, importance, and management. Archives of Pharmacal Research, 38 (7), 1281–1298. doi:10.1007/s12272–014–0517–z
Koiro, O. O. (2014). Rol biolohichno aktyvnykh rechovyn yahlytsi zvychainoi (Aegopodium podagraria L.) u nefroprotektornii, hepatoprotektornii ta hipoyrykemichnii dii. Kharkiv, 20.
Tovchiga, O.V. (2009). Doslidzhennia sechohinnoi, nefroprotektornii, hipoyrykemichnii dii yahlytsi zvychainoi (Aegopodium podagraria L.) yak osnova dlia stvorennia likarskikh zasobiv. Kharkiv, 21.
Tovchiga, O. (2014). Effects of Aegopodium podagraria Preparations on the Metabolic Disorders Induced in Rats by Excess Fructose Combined WITH Hydrochlorothiazide: The Relationship between Influence on Electrolyte and Carbohydrate Metabolism. International Journal of Biochemistry Research & Review, 4 (1), 80–98. doi: 10.9734/ijbcrr/2014/7201
Tovchiga, O. V. (2016). The influence of goutweed (Aegopodium podagraria L.) tincture and metformin on the carbohydrate and lipid metabolism in dexamethasone–treated rats. BMC Complementary and Alternative Medicine, 16 (1). doi: 10.1186/s12906–016–1221–y
Tovchiga, O. V. (2012). Farmakolohiia ta likarska toksykolohiia, 5, 73–78.
Stefanov, O. V. (ed.) (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: Avicenna, 528.
Tovchiga, O. V., Shtrygol, S. Yu., Stepanova, S. I. (2014). Use of 20% tincture of bishop’s–weed with 70% ethyl alcohol as means of hypoglycemic action. Pat. 104448 UA, ICP A61K 36/23 (2006.01), A61K 135/00, A61P 3/10 (2006.01); declared 25.07.11; published 10.02.2014, №3, 4.
Ratyni, O. A., Shtrygol, S. Yu., Slobodin, V. B. et al. (2000). Eksperimentalnaia i klinicheskaia farmakologiia, 63 (4), 42–44.
Koshevaia, Ye. Yu., Yakovleva, L. V., Bolokhovets, A. S., Kislichenko, V. S. (2009). Klinichna farmatsia, 1, 64–67.
Hunyadi, A., Martins, A., Hsieh, T.–J., Seres, A., Zupkó, I.(2012). Chlorogenic Acid and Rutin Play a Major Role in the In Vivo Anti–Diabetic Activity of Morus alba Leaf Extract on Type II Diabetic Rats. PLoS ONE, 7 (11), e50619. doi: 10.1371/journal.pone.0050619
Meng, S., Cao, J., Feng, Q., Peng, J., Hu, Y. (2013). Roles of Chlorogenic Acid on Regulating Glucose and Lipids Metabolism: A Review. Evidence–Based Complementary and Alternative Medicine, 2013, 801457. doi: 10.1155/2013/801457
Yamashita, R., Kikuchi, T., Mori, Y., Aoki, K., Kaburagi, Y., Yasuda, K., Sekihara, H. (2004). Aldosterone Stimulates Gene Expression of Hepatic Gluconeogenic Enzymes through the Glucocorticoid Receptor in a Manner Independent of the Protein Kinase B Cascade. Endocrine Journal, 51 (2), 243–251. doi: 10.1507/endocrj.51.243
Ferrannini, E., Galvan, A. Q., Santoro, D., Natali, A. (1992). Potassium as a link between insulin and the renin–angiotensin–aldosterone system. Journal of Hypertension, 10 (Supplement 1), S5–S10. doi: 10.1097/00004872–199204001–00002
Tovchiga, O. V. (2016). Interaction of Aegopodium podagraria L. (goutweed) with central nervous system depressants. Ukrainskyi biofarmatsevtychnyi zhurnal–Ukrainian biopharmaceutical journall, 1(42), 31–36.
Fodor, J., Grafnetter, D. (1960). The influence of thiopentone anaesthesia on the blood lipid and blood sugar level. British Journal of Pharmacology and Chemotherapy, 15 (2), 282–284. doi: 10.1111/j.1476–5381.1960.tb01245.x
Cardoso, A. R., Carvalho, C. R. O., Velloso, L. A., Brenelli, S. L., Saad, M. J. A., Carvalheira, J. B. C. (2005). Effect of thiopental, pentobarbital and diethyl ether on early steps of insulin action in liver and muscle of the intact rat. Life Sciences, 76 (20), 2287–2297. doi: 10.1016/j.lfs.2004.10.043
Bruno, C. M., Pricoco, G., Cantone, D. et al. (2016). Tubular handling of uric acid and factors influencing its renal excretion: a short review. EMJ Nephrol., 4 (1), 92–97. doi: 10.1186/1758–5996–4–12.
Cook, D. G., Shaper, A. G., Thelle, D. S., Whitehead, T. P. (1986). Serum uric acid, serum glucose and diabetes: relationships in a population study. Postgraduate Medical Journal, 62 (733), 1001–1006. doi: 10.1136/pgmj.62.733.1001
Jitapunkul, S., Chalaprawat, M., Bunnag, S. et al. (1991). The relationship between glucose and uric acid metabolism: influence of short term allopurinol on glucose metabolism. J. Med. Assoc. Thai, 74 (2), 80–86.
Lippi, G., Montagnana, M., Targher, G., Salvagno, G. L., Guidi, G. C. (2008). Relationship between uric acid, hyperglycemia and hypertriglyceridemia in general population. Biochemia Medica, 37–41. doi: 10.11613/bm.2008.005
Natarajan, V., Arul Gnana Dhas, A. S. (2013). Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin–induced diabetic rats. SpringerPlus, 2 (1), 394. doi: 10.1186/2193–1801–2–394
Prabhakar, P. K., Doble, M. (2009). Synergistic effect of phytochemicals in combination with hypoglycemic drugs on glucose uptake in myotubes. Phytomedicine, 16 (12), 1119–1126. doi: 10.1016/j.phymed.2009.05.021
Sudeep, H.V., Venkatakrishna, K., Dipak Patel, Shyamprasad, K. (2016). Biomechanism of chlorogenic acid complex mediated plasma free fatty acid metabolism in rat liver. BMC Complementary and Alternative Medicine, 16 (1), 274. doi: 10.1186/s12906–016–1258–y
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