The experimental study of the antihypoxic and antioxidant activity of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine derivatives

V. A. Volkovoy, O. V. Sevrukov, S. V. Kolisnyk, N. V. Derkach, S. I. Kryzhna, M. O. Ostapets

Abstract


Aim. To study the antihypoxic and antioxidant activity of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine derivatives on the models of the experimental pathology.
Materials and methods. The study of the antihypoxic activity of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine derivatives was conducted on the model of acute hemic hypoxia, the antioxidant action was studied on the model of toxic tetrachlorometane hepatitis.
Results and discussion. It was found that the activity of compounds KMS-161, KMS-164, KMS-166, KMS-168, KMS-174, KMS-176, KMS-191 was lower compared to mexidol, and the antihypoxic activity of compounds KMS-162, KMS-163, KMS-172, KMS-178, KMS-179, KMS-211, KMS-214, KMS-217 was close and slightly higher than the activity of mexidol in its median effective dose of 100 mg/kg. The marked antihypoxic activity was found in all groups studied. In the “structure – activity” analysis it was found that the antihypoxic activity among derivatives of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine was caused by their nature, the structure of the radical and the position of the substituent. The presence of the pyrrole ring in the molecule of 5,7-dihydro-1Н-pyrrolo-[2,3-d]pyrimidine led to the increased antihypoxic activity. A comparative analysis of the effectiveness of the objects studied showed that substances with the code of KMS-162, KMS-176, KMS-191, KMS-211, KMS-214, KMS-217 affected most positively on the state of AOS and LPO in conditions of acute hepatitis.
Conclusions. In a series of 5,7-dihydro-1Н-pyrrolo-[2,3-d]pyrimidine derivatives the most active was compound KMS-211, which showed the high antihypoxic activity (131 %) in relation to the control group and exceeded the reference drug mexidol by 45 %. Synthetic derivatives of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine exhibited the antioxidant activity (compound KMS-211 was the most active). The compounds studied may be the basis for the purposeful synthesis of antihypoxants and antioxidants.


Keywords


derivatives of 5,7-dihydro-1H-pyrrolo-[2,3-d]pyrimidine; hypoxia; antihypoxic action; lipid peroxidation; hepatitis; antioxidant activity

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DOI: https://doi.org/10.24959/nphj.17.2173

Abbreviated key title: Vìsn. farm.

ISSN 2415-8844 (Online), ISSN 1562-7241 (Print)