The study of biologically active substances of the extract obtained from peach leaves of the tajik variety

G. F. Navruzova, V. S. Kyslychenko, L. V. Lenchyk

Abstract


Aim. To study the qualitative composition and the content of biologically active substances (BAS) in the extract obtained from peach leaves grown in Tajikistan, its numerical parameters and choose the parameters for standardization of the extract.
Materials and methods. The object of the study was a soft extract obtained from peach leaves of the “Salvey” variety.
For standardization the numerical parameters were determined in 5 batches of the extract. A dry residue, the content of heavy metals and microbiological purity were studied in the extract by the methods of the State Pharmacopoeia of Ukraine (SPhU). The qualitative reaction and thin-layer chromatography (TLC) were proposed for identification of the extract. The content of the amount of phenolic compounds, flavonoids and hydroxycinnamic acids was determined using spectrophotometry calculated with reference to gallic acid, rutin and chlorogenic acid, respectively. The content of polysaccharides was determined gravimetrically, and the content of organic acids by titrimetry according to the SPhU monograph “Rosehips”.
Results and discussion. A dry residue of the extract was 78.27 ± 0.12 %. The content of heavy metals did not exceed 0.001 %. According to the results of microbiological purity determination the extract met the SPhU requirements. According to the results of the reaction with the solution of Ferrum (III) chloride the presence of phenolic substances was confirmed. During the TLC analysis several spots were observed, one of which appeared at the level of the standard sample of chlorogenic acid. The quantitative content (%) of the following substances was determined in the extract: polysaccharides – 14.74 ± 0.09, organic acids – 3.71 ± 0.09, phenolic compounds – 12.85 ± 0.39, flavonoids – 4.71 ± 0.09, hydroxycinnamic acids – 8.41 ± 0.07.
Conclusions. The analysis of BAS of a soft extract from peach leaves has been carried out. The parameters of its standardization have been selected.


Keywords


peach; leaves; extract; analysis; standardization

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References


FAO Statistical Pocketbook 2015. (2015). Rome: Food and Agriculture Organization of the United Nations. Available at: http://faostat3.fao.org/

Lenchik, L. V., Navruzova, G. F., Kislichenko, V. S., Sharipov, H. Sh., Zaichenko, A. V. (2014). Respublikanskii nauchnyi zhurnal

“Vestnik”, 3 (68), 126–132.

Lenchyk, L., Navruzova, G., Kyslychenko, V., Saidov, N. (2015). Study of phenolic compounds of peach leaves extract. American

Journal of science and Technologies, 2 (20), 795–799.

Kyslychenko, V., Lenchyk, L., Navruzova, G., Sharifov, Kh., Zaychenko, G. (2015). Study of Рersica vulgaris leaves as prospective

source of new phytomedicines. The 15 international Congress of the international society for Ethnopharmacology, 05–08 of May 2015.

Petra, 114.

Derzhavna Farmakopeia Ukrainy. (2nd ed.). (2015). Kharkiv: Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv, 723.

European Pharmacopoeia, 4th ed. (2002). Council of Europe. Strasburg, 2416.

Quality control methods for medicinal plant materials. (1998). World Health Organization. Geneva, 123.

Upyr, D. V., Martynov, A. V., Kyslychenko, V. S., Ilinska, N. I. (2013). Vìsnik farmacìï, 2 (74), 38–40.

Kovalev, V. N., Popova, N. V., Kislichenko, V. S. (2003). Praktikum po farmakognozii. Kharkiv: NUPh, Zolotye stranitcy, 512.


GOST Style Citations


1. FAO Statistical Pocketbook 2015. – Rome : Food and Agriculture Organization of the United Nations, 2015. – Available at : http://
faostat3.fao.org/

2. Фитохимическое и фармакологическое изучение листьев Persica vulgaris, заготовленных в Таджикистане / Л. В. Ленчик, Г. Ф. Наврузо-
ва, В. С. Кисличенко и др. // Республиканский научный журн. “Vestnik”. – № 3 (68), Том IV. – Шимкент : Серпилис, 2014. –
С. 126–132.

3. Study of phenolic compounds of peach leaves extract / L. Lenchyk, G. Navruzova, V. Kyslychenko, N. Saidov // Am. J. of Sci. and
Technol. – 2015. – Vol. 2, Issue 20. – P. 795–799.

4. Study of Рersica vulgaris leaves as prospective source of new phytomedicines / V. S. Kyslychenko, L. V. Lenchyk, G. F. Navruzova et al. //
The 15 International Congress of the International Society for Ethnopharmacology, 05–08 of May 2015. – Petra, Jordan, 2015. – 114 p.

5. Державна фармакопея України / Державне підприємство «Український науковий фармакопейний центр якості лікарських засо-
бів». – 2–е вид. – Х. : Український науковий фармакопейний центр якості лікарських засобів, 2014. – 732 с.

6. European Pharmacopoeia, 4-th ed. – Council of Europe, Strasburg. – 2002. – 2416 p.

7. Quality control methods for medicinal plant materials. – World Health Organization. – Geneva, 1998. – 123 р.

8. Стандартизація збору для лікування інфекційних захворювань легень і туберкульозу / Д. В. Упир, А. В. Мартинов, В. С. Кис-
личенко, Н. І. Ільїнська // Вісник фармації. – 2013.– № 2 (74). – С. 38–40.

9. Практикум по фармакогнозии : учеб. пособие для студ. вузов / В. Н. Ковалев, Н. В. Попова, В. С. Кисличенко и др. ; под. общ.
ред. В. Н. Ковалева. – Х. : НФаУ; Золотые страницы, 2003. – 512 с.





DOI: https://doi.org/10.24959/nphj.17.2178

Abbreviated key title: Vìsn. farm.

ISSN 2415-8844 (Online), ISSN 1562-7241 (Print)