Development of a novel kinetic spectrophotometric method for the quantitative determination of Cefuroxime using Caro’s acid

Authors

  • Yu. Yu. Serdiukova National University of Pharmacy, Ukraine http://orcid.org/0000-0002-4755-3660
  • S. G. Leonova National University of Pharmacy, Ukraine
  • Yu. S. Kolisnyk National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/nphj.17.2181

Keywords:

Cefuroxime, kinetic spectrophotometric method, quantitative determination, Caro’s acid

Abstract

Development of new simple and economically profitable methods for the quantitative determination of Cefuroxime substance is of a great interest.

Aim. To study the reaction of Cefuroxime with Caro’s acid and develop a new method for the quantitative determination of Cefuroxime based on the product of S-oxidation and perhydrolysis.

Materials and methods. To determine Cefuroxime substance the triple potassium salt of Caro’s acid was used as an oxidizing agent. The procedure was developed as a kinetic spectrophotometric method.

Results and discussion. The initial rate method can be easily applied to determine Cefuroxime substance. The data obtained showed good results in accuracy, precision, LOQ and LOD. The RSD for the substance was 1.53-2.35 %, δ = 0.55-1.40 %. The direct linear dependence was observed in a wide range of concentrations 1-7 µg·mL-1.

Conclusions. The possibility of Cefuroxime analytical determination by the biologically active part of the molecule, reproducible results and accuracy are the advantages of the method proposed. The data obtained are in good agreement with the standard pharmacopoeial HPLC method. 

Author Biographies

Yu. Yu. Serdiukova, National University of Pharmacy

Candidate of Pharmacy (Ph. D.), teaching assistant of the Department of Physical and Colloid Chemistry

S. G. Leonova, National University of Pharmacy

Candidate of Pharmacy (Ph. D.), associate professor of the Pharmaceutical Chemistry Department

Yu. S. Kolisnyk, National University of Pharmacy

Candidate of Pharmacy (Ph. D.), teaching assistant of the Department of Analytical Chemistry

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Published

2017-12-04

Issue

Section

Synthesis and Analysis of Biologically Active Substances