Development of the methods for the quantitative determination of fusidic acid and panthenol in the composition of “Fuzipam-derma” dermatological gel

Authors

  • P. P. Baiva National University of Pharmacy, Ukraine https://orcid.org/0000-0003-0408-0776
  • S. M. Kovalenko National University of Pharmacy, Ukraine
  • I. I. Baranova National University of Pharmacy, Ukraine
  • S. O. Mamedova National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/nphj.18.2197

Keywords:

acne, gel, chromatogram, panthenol, fusidic acid

Abstract

Aim. To develop and validate the methods for the quantitative determination of fusidic acid and panthenol in “Fuzipam-derma” gel.

Materials and methods. The determination was performed by high performance liquid chromatography (HPLC) according to the requirements of the State Pharmacopoeia of Ukraine (SPhU) 1.0 on a Prostar-210 liquid chromatograph, (Varian Chromatography System, USA).

Results and discussion. The methods for the quantitative determination of fusidic acid and panthenol in “Fuzipam-derma” gel have been developed. The appropriate chromatographic conditions have been chosen, due to them the peaks of fusidic acid and panthenol are completely separated from other gel components. The validation of the methods for the quantitative determination of fusidic acid and panthenol has been performed. The data obtained have shown that the methods are stable and reproducible in different days.

Conclusions. The methods for the quantitative determination of fusidic acid and panthenol in the composition of the new dermatological gel “Fuzipam-derma” for the treatment of grade І-ІІ acne has been developed using the HPLC method.

References

  1. Taylor, G. A., Shalita, A. R. (2004). Benzoyl Peroxide–Based Combination Therapies for Acne Vulgaris. American Journal of Clinical Dermatology, 5 (4), 261–265. doi: 10.2165/00128071–200405040–00005
  2. Eady, E. A., Gloor, M., Leyden, J. J. (2003). Propionibacterium acnes Resistance: A Worldwide Problem. Dermatology, 206 (1), 54–56. doi: 10.1159/000067822
  3. Brüggemann, H. (2010). Skin: Acne and Propionibacterium acnes Genomics. Handbook of Hydrocarbon and Lipid Microbiology, 3215–3225. doi: 10.1007/978–3–540–77587–4_244
  4. Dashkova, N. A., Logachev, M. F. (2006). Vestnik dermatologii i venerologii, 4, 8–11.
  5. Bolotnaia, L. A., Serbina, I. M., Ovcharenko, Yu. S. (2009). Novosti meditciny i farmatcii. Dermatologiia i kosmetologiia, 76–86.
  6. Dvornikova, A. S., Kruglova, L. S. (2007). Klinicheskaia dermatologiia i venerologiia, 6, 19–23.
  7. Baiva, P. P., Baranova, I.I.(2012). Actualni pytannia stvorennia novykh likarskykh zasobiv. Kharkiv, 425.
  8. Baiva, P. P., Baranova, I.I.(2012). Suchasni dosiahnennia farmatsevtychnoi tekhnolohii. Kharkiv, 10–11.
  9. Baiva, P. P., Baranova, I.I.(2012). Molod – medytsyni maibutnoho. Odessa, 95–96.
  10. Baiva, P. P., Baranova, I. I., Kovalova, T. M. (2011). Suchasni dosiahnennia farmatsevtychnoi tekhnolohii. Kharkiv, 133.
  11. Baiva, P. P., Baranova, I. I. (2013). Tovaroznavchi aspekty spozhyvchykh tovariv. Kharkiv, 67–68.
  12. Derzhavna Farmakopeia Ukrainy, 1–e vyd. (2001). Kharkiv: RIREG.
  13. Weiss, J. (2005). Handbook of Ion Chromatography.New York: John Wiley & Sons, 931.
  14. Kazakevich, Y. V. (2007) HPLC for Pharmaceutical Scientists, 1140.
  15. Cazes, J. (2009). Encyclopedia of chromatography.New York: CRC Press, 2850.
  16. Derzhavna Farmakopeia Ukrainy 1–e vyd., 2–e dop. (2008). Kharkiv: RIREG.
  17. Bliesner, D. (2006). Validating Chromatographic Methods: A Practical Guide.London;New York: John Wiley & Sons, 304.

Downloads

Published

2018-03-15

Issue

No. 1(93) (2018)

Section

Synthesis and Analysis of Biologically Active Substances

License

Copyright (c) 2018 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.