Development of the methods for the quantitative determination of fusidic acid and panthenol in the composition of “Fuzipam-derma” dermatological gel

Authors

  • P. P. Baiva National University of Pharmacy, Ukraine https://orcid.org/0000-0003-0408-0776
  • S. M. Kovalenko National University of Pharmacy, Ukraine
  • I. I. Baranova National University of Pharmacy, Ukraine
  • S. O. Mamedova National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/nphj.18.2197

Keywords:

acne, gel, chromatogram, panthenol, fusidic acid

Abstract

Aim. To develop and validate the methods for the quantitative determination of fusidic acid and panthenol in “Fuzipam-derma” gel.

Materials and methods. The determination was performed by high performance liquid chromatography (HPLC) according to the requirements of the State Pharmacopoeia of Ukraine (SPhU) 1.0 on a Prostar-210 liquid chromatograph, (Varian Chromatography System, USA).

Results and discussion. The methods for the quantitative determination of fusidic acid and panthenol in “Fuzipam-derma” gel have been developed. The appropriate chromatographic conditions have been chosen, due to them the peaks of fusidic acid and panthenol are completely separated from other gel components. The validation of the methods for the quantitative determination of fusidic acid and panthenol has been performed. The data obtained have shown that the methods are stable and reproducible in different days.

Conclusions. The methods for the quantitative determination of fusidic acid and panthenol in the composition of the new dermatological gel “Fuzipam-derma” for the treatment of grade І-ІІ acne has been developed using the HPLC method.

Author Biographies

P. P. Baiva, National University of Pharmacy

postgraduate student of the Department of Cosmetology and Aromology

S. M. Kovalenko, National University of Pharmacy

Doctor of Pharmacy (Dr.habil), рrofessor of the Department of Commodity Science

I. I. Baranova, National University of Pharmacy

д-р фарм. наук, професор, завідувачка кафедри товарознавства

S. O. Mamedova, National University of Pharmacy

канд. фарм. наук, асистент кафедри товарознавства

References

Taylor, G. A., Shalita, A. R. (2004). Benzoyl Peroxide–Based Combination Therapies for Acne Vulgaris. American Journal of Clinical Dermatology, 5 (4), 261–265. doi: 10.2165/00128071–200405040–00005

Eady, E. A., Gloor, M., Leyden, J. J. (2003). Propionibacterium acnes Resistance: A Worldwide Problem. Dermatology, 206 (1), 54–56. doi: 10.1159/000067822

Brüggemann, H. (2010). Skin: Acne and Propionibacterium acnes Genomics. Handbook of Hydrocarbon and Lipid Microbiology, 3215–3225. doi: 10.1007/978–3–540–77587–4_244

Dashkova, N. A., Logachev, M. F. (2006). Vestnik dermatologii i venerologii, 4, 8–11.

Bolotnaia, L. A., Serbina, I. M., Ovcharenko, Yu. S. (2009). Novosti meditciny i farmatcii. Dermatologiia i kosmetologiia, 76–86.

Dvornikova, A. S., Kruglova, L. S. (2007). Klinicheskaia dermatologiia i venerologiia, 6, 19–23.

Baiva, P. P., Baranova, I.I.(2012). Actualni pytannia stvorennia novykh likarskykh zasobiv. Kharkiv, 425.

Baiva, P. P., Baranova, I.I.(2012). Suchasni dosiahnennia farmatsevtychnoi tekhnolohii. Kharkiv, 10–11.

Baiva, P. P., Baranova, I.I.(2012). Molod – medytsyni maibutnoho. Odessa, 95–96.

Baiva, P. P., Baranova, I. I., Kovalova, T. M. (2011). Suchasni dosiahnennia farmatsevtychnoi tekhnolohii. Kharkiv, 133.

Baiva, P. P., Baranova, I. I. (2013). Tovaroznavchi aspekty spozhyvchykh tovariv. Kharkiv, 67–68.

Derzhavna Farmakopeia Ukrainy, 1–e vyd. (2001). Kharkiv: RIREG.

Weiss, J. (2005). Handbook of Ion Chromatography.New York: John Wiley & Sons, 931.

Kazakevich, Y. V. (2007) HPLC for Pharmaceutical Scientists, 1140.

Cazes, J. (2009). Encyclopedia of chromatography.New York: CRC Press, 2850.

Derzhavna Farmakopeia Ukrainy 1–e vyd., 2–e dop. (2008). Kharkiv: RIREG.

Bliesner, D. (2006). Validating Chromatographic Methods: A Practical Guide.London;New York: John Wiley & Sons, 304.

Downloads

Published

2018-03-15

Issue

No. 1(93) (2018)

Section

Synthesis and Analysis of Biologically Active Substances

License

Copyright (c) 2018 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).