Development of methods for identification and quantitative determination of active substances in the medicated cosmetic cream for use in trichology
Keywords:androgenic alopecia, cream, chemical analysis, quality parameters
To prevent and treat androgenic alopecia (AA) a medicated cosmetic cream (MCC) with the saw palmetto extract and the Sophora japonica tincture has been developed. The introduction of MCC in production requires its standardization and development of its quality control methods.
Aim. To determine the qualitative composition and quantitative content of biologically active substances (BAS) in MCC for prevention and complex treatment of AA.
Materials and methods. The study object was the experimental MCC with the saw palmetto extract and the Sophora japonica tincture. The qualitative reactions and the method of adsorption spectrometry (MAS) were proposed for BAS identification and quantitative determination.
Results and discussion. During the study flavonoids and phytosterols were identified. The content of flavonoids calculated with reference to rutin was determined using MAS, it was 1.85 mg/g of MCC. The quantitative content of the amount of triglycerides and phytosterols calculated with reference to β-amirine was 3.59 mg/g of MCC.
Conclusions. The qualitative reactions have been chosen to identify flavonoids for the Sophora japonica tincture and phytosterols for the saw palmetto extract; the conditions for identification and quantitative determination of BAS by MAS have been proposed. The methods developed will be used in the draft of Quality Control Methods (QCM) for MCC for prevention and complex treatment of AA.
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