The comparative study of flavonoids in pear leaves of Noyabrska, Forest beauty and Lemonka varieties
Keywords:ordinary pear, flavonoids, qualitative and quantitative analysis, spectrophotometry
Aim. To study the qualitative composition and determine the quantitative content of flavonoids in pear leaves of Noyabrska, Forest beauty and Lemonka varieties.
Materials and methods. As objects of research, leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties were selected. Identification of flavonoids was performed using qualitative reactions and thin-layer chromatography. The quantitative content was determined by the spectrophotometric method.
Results and discussion. The presence of flavonoids in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties was found by qualitative reactions. Using thin-layer chromatography the following components were identified in leaves of pears of the varieties studied: among glycosides – kosmosien, cynaroside, astragalin, rutin, hyperoside, quercitrin, isoquercitrin, among aglycons – apigenin, luteolin, kaempferol, quercetin. The content of flavonoids calculated with reference to rutin in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties was 2.88 ± 0.07%, 1.31 ± 0.03% and 1.64 ± 0.03%, respectively.
Conclusions. The study of the qualitative composition and quantitative content of flavonoids in leaves of ordinary pears of Noyabrska, Forest beauty and Lemonka varieties has shown that they are not inferior to wild pear trees and can be an additional source of flavonoids for the purpose of creating herbal medicines on their basis.
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