The analysis of serotonin derivatives from safflower seeds


  • N. V. Popova National University of Pharmacy, Ukraine
  • V. I. Litvinenko State Scientific Center of Medicines, Ukraine
  • O. V. Kaidanska National University of Pharmacy, Ukraine
  • M. M. Boyko Belgorod National State University, Russian Federation
  • D. A. Alferova National University of Pharmacy, Ukraine



safflower, N-feruloylserotonin, N-cumaroylserotonin


Safflower, or American saffron, or wild saffron, or thistle (Carthamus tinctorius L.) is found both in the wild form as a weed and in the cultivated form. About 200 chemical components have been isolated from various organs of safflower, including coumarins, flavonoids, steroids, glycosides, pigments and fatty oils. To date, safflower is used as a dietary supplement with the antidiabetic, antihypertensive, antioxidant, anti-inflammatory, reparative and other types of activity. Taking into account the above-mentioned properties of the plant further pharmacognostic studies of safflower is relevant.

Aim. To analyze serotonin derivatives in domestic varieties of safflower.

Materials and methods. For the preliminary analysis of serotonin derivatives in the alcoholic extract from safflower seeds the paper and thin layer chromatographies (TLC and PC) were used. For further studies of the chemical composition of the alcoholic extract obtained the high-performance liquid chromatography reversed-phase, UV- and mass-spectroscopy were used.

Results and discussion. Using the preliminary chromatographic analysis (TLC and PC) of the safflower seed extracts two substances with an indole heterocycle were identified. On the chromatogram of the HPLC method two dominant substances were detected, for which the UV- and mass-spectra were determined. These compounds were identified as N-feruloylserotonin and N-cumaroylserotonin.

Conclusions. For the first time serotonin derivatives have been identified from seeds of domestic varieties of safflower.

Author Biographies

N. V. Popova, National University of Pharmacy

Doctor of Pharmacy (Dr. habil.), professor, head of the Department of Nutritiology and Pharmaceutical Bromatology

V. I. Litvinenko, State Scientific Center of Medicines

Doctor of Chemistry (Dr. habil.), professor, head of the Sector of Chemistry and Technology of Phenolic Medicines, State Scientific Center of Medicines

O. V. Kaidanska, National University of Pharmacy

postgraduate student of the Department of Nutritiology and Pharmaceutical Bromatology

M. M. Boyko, Belgorod National State University

Candidate of Pharmacy (Ph.D.), associate professor of the Pharmaceutical Technology Department

D. A. Alferova, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), associate professor of the Department of Nutritiology and Pharmaceutical Bromatology


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