Development of quality control methods of active ingredients of the gel with the venotonic action
Keywords:Gel, liquid extract from Aesculus hippocastanum seeds, liquid extract from Delphinium elatum roots, quality control methods
Aim. To develop quality control methods of biologically active substances of liquid extracts from Aesculus hippocastanum seeds and Delphinium elatum roots, which are the main components of the new dosage form in the form of the gel with the venotonic action.
Materials and methods. Identification of the main biologically active substances of plant extracts was carried out by chemical reactions and thin layer chromatography in the solvent system of chloroform – glacial acetic acid – methanol – water (15 : 8 : 3 : 2) using standard samples of escin and allantoin as reference samples. Detection of chromatograms was performed by treating with phosphatotungstic acid solution. The quantitative determination was carried out by absorption spectrophotometry in the visible range. Calculation of the quantitative content was performed by the standard method.
Results and discussion. By chemical reactions biologically active substances of polyphenolic structure were found in the gel studied. The presence of escin and allantoin in the gel composition was confirmed by the method of thin layer chromatography compared to standard samples. The quantitative determination of the amount of triterpene saponins calculated with reference to escin was performed by spectrophotometry in the visible range, and it was from 32 mg to 42 mg in the gel.
Conclusions. The results obtained when performing chemical reactions, the method of thin-layer chromatography and the quantitative determination by absorption spectrophotometry allow recommending the dosage form studied for standardization by the amount of triterpene saponins calculated with reference to escin.
Hontova, T. M. (2013). Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 2 (12), 4–6.
Derzhavna Farmakopeia Ukrainy. (2015). DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 2–e vyd., Kharkiv: DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», T.1, 1128.
Rybachenko, A. Y., Bashura, A. H. (1986). Khromato-spektrofotometrycheskoe opredelenye saponyna estsyna v aerozolnom preparate. Kazan, 321.
Tkacheva, O. V., Yakovleva, L. V., Grubnyk Y. M. (2013). Razrabotka y rehystratsyia lekarstvennykh sredstv, 2, 66–69.
Abudayeh, Z. H. M., Al Azzam, K. M., Naddaf, A., Karpiuk, U. V., & Kislichenko, V. S. (2015). Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography. Advanced Pharmaceutical Bulletin, 5 (4), 587–591. https://doi.org/10.15171/apb.2015.079
Neagu, E., Paun, G., Radu L. G. (2011). Phytochemical study of some Symphytum officinale extracts concentrated by membranous procedures. Sci. Bull., Series B., 73 (3), 65–74.
De Almeida, P. A., Alves, M. C., Polonini H. C., … Dutra L. S. (2013). New HPLC method for quality control of β-escin in Aesculus hippocastanum L. hydroalcoholic extract. Lat. Am. J. Pharm., 32 (7), 1082–1087.
Vujic, Z., Novovic, D., Ascis, I. (2013). Antic Optimization of extraction process of escin from dried seeds of Aesculus hippocastanum L. by Derringer’s desirability function. Journal of Animal&Plant Sciences, 17 (1), 2514–2521.
Trifan, A., Opitz, S. E. W., Josuran, R., Grubelnik, A., Esslinger, N., Peter, S., … Wolfram, E. (2018). Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots. Food and Chemical Toxicology, 112, 178–187. https://doi.org/10.1016/j.fct.2017.12.051.
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