The kinetic spectrophotometric method for the determination of azlocillin in solutions


  • S. P. Karpova National University of Pharmacy, Ukraine
  • M. Ye. Blazheyevskiy National University of Pharmacy, Ukraine
  • O. O. Mozgova National University of Pharmacy, Ukraine
  • M. M. Ivashura National University of Pharmacy, Ukraine



oxidation, azlocillin, validation, potassium peroxomonosulphate, spectrophotometry


Aim. To develop the method for the quantitative determination of azlocillin.

Materials and methods. The study object was Securopen® – a powder of azlocillin (Azl) sodium in vials for preparation of the solution for injections (Azlocillin, 1.0 g). Peroxomonosulfate acid as triple potassium salt 2КНSO5 ∙ КНSO4 ∙ K2SO4 (Oxone®) of “extra pure” qualification was used as an oxidant.

Results and discussion. The kinetics of the conjugated reactions of S-oxidation and perhydrolysis of Azlocillin (Azl) with potassium peroxomonosulfate in the alkaline medium has been studied by the increase of the forming product light absorbance at 275 nm. The conditions have been optimized, and the procedure of the quantitative analysis of Azl by the kinetic spectrophotometric method has been developed using potassium peroxomonosulfate as a reagent. RSD = 2.02 %.

Conclusions. The results of the drug analysis obtained by the developed and standard methods are in good agreement with each other; δ = +0.49 %.


Author Biographies

S. P. Karpova, National University of Pharmacy

Candidate of Pharmacy (Ph. D), associate professor of the Physical and Colloid Chemistry Department

M. Ye. Blazheyevskiy, National University of Pharmacy

Doctor of Chemistry (Dr. habil), professor of the Physical and Colloid Chemistry Department

O. O. Mozgova, National University of Pharmacy

Candidate of Pharmacy (Ph. D), teaching assistant of the Physical and Colloid Chemistry Department

M. M. Ivashura, National University of Pharmacy

Candidate of Agricultural Sciences (Ph. D), teaching assistant of the Physical and Colloid Chemistry Department


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