The kinetic spectrophotometric method for the determination of azlocillin in solutions
DOI:
https://doi.org/10.24959/nphj.19.2247Keywords:
oxidation, azlocillin, validation, potassium peroxomonosulphate, spectrophotometryAbstract
Aim. To develop the method for the quantitative determination of azlocillin.
Materials and methods. The study object was Securopen® – a powder of azlocillin (Azl) sodium in vials for preparation of the solution for injections (Azlocillin, 1.0 g). Peroxomonosulfate acid as triple potassium salt 2КНSO5 ∙ КНSO4 ∙ K2SO4 (Oxone®) of “extra pure” qualification was used as an oxidant.
Results and discussion. The kinetics of the conjugated reactions of S-oxidation and perhydrolysis of Azlocillin (Azl) with potassium peroxomonosulfate in the alkaline medium has been studied by the increase of the forming product light absorbance at 275 nm. The conditions have been optimized, and the procedure of the quantitative analysis of Azl by the kinetic spectrophotometric method has been developed using potassium peroxomonosulfate as a reagent. RSD = 2.02 %.
Conclusions. The results of the drug analysis obtained by the developed and standard methods are in good agreement with each other; δ = +0.49 %.
References
Alekseev, V. G., Lapshin, S. V. (2007). Voprosy biologicheskoi, meditcinskoi i farmatcevticheskoi khimii, 1, 27–30.
Blazheievskyi, M. Ye. (2005). Ukrainskyi khimichnyi zhurnal, 71 (10), 90–93.
3. U.S. Pharmacopoeia 30–NF25, National Formulary 25. (2008). Pharmacopoeial Convention :Rockville, 2137.
Karpova, S. P., Blazheyevskiy, M. Y., Mozgova, O. O. (2018). Development and validation of uv spectrophotometric area under curve method quantitative estimation of piperacillin. International Journal of Pharmaceutical Sciences and Research, 9 (8), 3556–3560.
Evaggelopoulou, E. N., & Samanidou, V. F. (2013). Development and validation of an HPLC method for the determination of six penicillin and three amphenicol antibiotics in gilthead seabream (Sparus Aurata) tissue according to the European Union Decision 2002/657/EC. Food Chemistry, 136 (3–4), 1322–1329. https://doi.org/10.1016/j.foodchem.2012.09.044
Karpova, S. P., Blazheyevskiy, M. Ye., Serdiukova, Y. Yu., Mozgova, O. O. (2018). Quantitative determination of azlocillin by iodometric method using potassium peroxomonosulfate. Asian Journal of Pharmaceutics, 12 (2), 508–511. https://doi.org/10.22377/ajp.v12i02.2383
Liu, K., Sun, D., Zhao, Y. (2008). Assay detection for azlocillin sodium and sulbactam sodium for injection by HPLC. Chin. J. Pharm. Anal, 28 (9), 1568–1570.
Khare, B., Khare, K. (2017). Spectrophotometric determination of antibiotic drug penicillin in pharmaceutical samples using 2,6 dichlorophenol indophenol, N–bromocaprolactam and N–chlorosuccinimide. Int. J. Rec. Res. Phys. Chem. Sci, 4, 1–7.
Singh, D. K., & Maheshwari, G. (2010). Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin–Ciocalteu reagent. Drug Testing and Analysis, 2 (10), 503–506. https://doi.org/10.1002/dta.157
Sallach, J. B., Snow, D., Hodges, L., Li, X., & Bartelt–Hunt, S. (2015). Development and comparison of four methods for the extraction of antibiotics from a vegetative matrix. Environmental Toxicology and Chemistry, 35 (4), 889–897. https://doi.org/10.1002/etc.3214
Švorc, Ľ., Sochr, J., Rievaj, M., Tomčík, P., & Bustin, D. (2012). Voltammetric determination of penicillin V in pharmaceutical formulations and human urine using a boron–doped diamond electrode. Bioelectrochemistry, 88, 36–41. https://doi.org/10.1016/j.bioelechem.2012.04.004" target="_blank">https://doi.org/10.1016/j.bioelechem.2012.04.004
Dubenska, L., Blazhejevskyj, M., Plotycya, S. (2017). Voltammetric methods for the determination of pharmaceuticals. J. Meth. Obj. Chem. Anal, 12, 61–75.
Hubicka, U., Krzek, J., Woltynska, H. (2009). Simultaneous identification and quantitative determination of selected aminoglycoside antibiotics by thin–layer chromatography and densitometry. J. AOAC Int, 92, 1068–1075.
Blazheyevskiy, М. Ye., Mozgova, O. O. (2015). Voltammetric determination of magnesium monoperoxyphthalate in pure substance and disinfectant “Dismozon pur”. Manag. Econom. Qual. Assurance. Pharm, 4, 4–11.
Baek, J. H., Choi, M. G., Kim, D. B., Kim, N. Y., Kang, E., Ahn, S., & Chang, S.–K. (2018). Fluorescence sensing of peracetic acid by oxidative cleavage of phenylselenyl ether of 4-hydroxynaphthalimide. Tetrahedron Letters, 59 (13), 1254–1257. https://doi.org/10.1016/j.tetlet.2018.02.048" target="_blank">https://doi.org/10.1016/j.tetlet.2018.02.048
Downloads
Published
Issue
Section
License
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
