DOI: https://doi.org/10.24959/nphj.20.23

Determination of L-Cystine in Tablets Using the Chemiluminescence Method

M. Ye. Blazheyevskiy, N. Yu. Bondarenko, Yu. Yu. Serdiukova, V. D. Yaremenko

Abstract


Aim. To develop the method for the quantitative determination of L-cystine in sublingual tablets Elthacin® using the method of chemiluminescence inhibition in the H2L (luminol) – Н2О2 – Нb (hemoglobin) system.

Materials and methods. The study objects were the pure substance of L-cystine of pharmaceutical grade and sublingual tablets Elthacin® produced by the Medical Scientific-Production Complex “Biotics Ltd” (Russia) containing 70 mg of glycine, L-glutaminic acid and L-cystine in their composition. The intensity of chemiluminescence was measured on the device with a FEU-84-A photoelectric multiplier using an IMT-0.5 measuring instrument of low currents and a quick-acting automatic potentiometer.

Results and discussion. The method of the L-cystine quantitative determination in tablets based on the inhibition of chemiluminescence in the H2L – H2O2 – Hb system has been developed. The calibration graph was linear over the concentration range from 7 to 70 μg · mL–1. No interferences were observed in the presence of common components of the tablets, such as glycine and L-glutaminic acid. RSD = ± 2.35 %, (δ = + 1.13 %), LOD (3S) = 4 μg · mL–1, LOQ (10S) = 13 μg · mL–1 for the sublingual tablets Elthacin®.

Conclusions. The method proposed is promising for further research on the subject of its application for the determination of L-cystine in drugs.

Keywords


L-cystine; inhibition; luminol; chemilumeniscence; hemoglobin

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References


Blazheyevskiy, M. Y., & Moroz, V. P. (2018). Iodometric determination of cystine in pharmaceutical formulation using diperoxyadipic acid as reagent. Methods and objects of chemical analysis, 13 (1), 13–17. https://doi.org/10.17721/moca.2018.13-17

Damle, M. S., Newton, L. A. A., Villalba, M. M., Leslie, R., & Davis, J. (2010). Plumbagin: A New Route to the Electroanalytical Determination of Cystine. Electroanalysis, 22 (21), 2491–2495. https://doi.org/10.1002/elan.201000198

Shukla, J., & Pitre, K. . (2002). Analysis of cystine in human blood for monitoring of cases of burns. Journal of Pharmaceutical and Biomedical Analysis, 27 (5), 821–826. https://doi.org/10.1016/s0731-7085(01)00486-1

Shaidarova, L. G., Ziganshina, S. A., Tikhonova, L. N., & Budnikov, G. K. (2003). Electrocatalytic Oxidation and Flow-Injection Determination of Sulfur-Containing Amino Acids at Graphite Electrodes Modified with a Ruthenium Hexacyanoferrate Film. Journal of Analytical Chemistry, 58 (12), 1144–1150. https://doi.org/10.1023/b:janc.0000008953.67244.c2

Shpak, A. V., Pirogov, A. V., Shpigun, О. A. (2003). Determination of Amino Acids by Capillary Electrophoresis without Preliminary Derivatization. Zhurnal analiticheskoi khimii, 58 (7), 317–319.

Ensafi, A. A., Rezaei, B., & Nouroozi, S. (2009). Flow injection spectrofluorimetric determination of cystine and cysteine. Journal of the Brazilian Chemical Society, 20 (2), 288–293. https://doi.org/10.1590/s0103-50532009000200013

Bhawani, S. A., Mohamad Ibrahim, M. N., Sulaiman, O., Hashim, R., Mohammad, A., & Hena, S. (2012). Thin-layer chromatography of amino acids: a review. Journal of Liquid Chromatography & Related Technologies, 35 (11), 1497–1516. https://doi.org/10.1080/10826076.2011.619039

Jamalpoor, A., Sparidans, R. W., Pou Casellas, C., Rood, J. J. M., Joshi, M., Masereeuw, R., & Janssen, M. J. (2018). Quantification of cystine in human renal proximal tubule cells using liquid chromatography-tandem mass spectrometry. Biomedical Chromatography, 32 (8), e4238. https://doi.org/10.1002/bmc.4238

Cebi, N., Dogan, C. E., Develioglu, A., Yayla, M. E. A., & Sagdic, O. (2017). Detection of l-Cysteine in wheat flour by Raman microspectroscopy combined chemometrics of HCA and PCA. Food Chemistry, 228, 116–124. https://doi.org/10.1016/j.foodchem.2017.01.132

10. European Pharmacopoeia 9.0. (2016). Cystine. Council of Europe:Strasbourg, 2187–2188.

Chernobrovkin, M. G., Koltcova, N. V., Shepelev, B. N. (2004). Farmatciia, 5, 18–20.

Blazheyevskiy, M. Ye., Riabko, D. N. (2014). Application of peroxy acids as disinfectants and sterilization agents (monograph). Saarbrücken (Deutschland): LAP LAMBERT Academic Publishing.


GOST Style Citations


1. Blazheyevskiy, M. Y. Iodometric determination of cysteine in pharmaceutical formulation using diperoxyadipinic acid as reagent / M. Y. Blazheyevskiy, V. P. Moroz // Methods and objects of chemical analysis. – 2018. – № 13 (1). – P. 13–17. https://doi.org/10.17721/moca.2018.13-17 

 

2. A New Route to the Electroanalytical Determination of Cystine / M. S. Damle, L. A. A. Newton, M. M. Villalba et al. // Electroanalysis. – 2010. – № 22 (21). – P. 2491–2495. https://doi.org/10.1002/elan.201000198

 

3. Shukla, J. Analysis of cystine in human blood for monitoring of cases of burns / J. Shukla, K. S. Pitre // J. Pharm. and Biomed. Anal. – 2002. – № 27 (5). – P. 821–826. https://doi.org/10.1016/s0731-7085(01)00486-1 

 

4. Electrocatalytic oxidation and flow-injection determination of sulfur-containing amino acids at graphite electrodes modified with a ruthenium hexacyanoferrate film / L. G. Shaidarova, S. A. Ziganshina, L. N. Tikhonova, G. K. Budnikov // J. of Analytical Chem. – 2003. – № 58 (12). – P. 1144–1150. https://doi.org/10.1023/b:janc.0000008953.67244.c2

 

5. Шпак, А. В. Определение аминокислот методом капиллярного электрофореза без предварительной дериватизации / А. В. Шпак, А. В. Пирогов, О. А. Шпигун // Журн. аналит. химии. – 2003. – T. 58, № 7. – С. 317–319.

 

6. Ensafi, A. A. Flow injection spectrofluorimetric determination of cystine and cysteine / A. A. Ensafi, B. Rezaei,S. Nouroozi// J. Braz. Chem. Soc. – 2009. – № 20 (2). – P. 288–293. https://doi.org/10.1590/s0103-50532009000200013 

 

7. Thin-layer chromatography of amino acids / S. A. Bhawani, M. N. Mohamad Ibrahim, O. Sulaiman et al. // J. Liq. Chrom. & Rel. Tech. – 2012. – № 35 (11). – P. 1497–1516. https://doi.org/10.1080/10826076.2011.619039 

 

8. Quantification of cysteine in human renal proximal tubule cells using liquid chromatography – tandem mass spectrometry / A. Jamalpoor, R. W. Sparidans, C. P. Casellas et al. // Biomed. Chromatogr. – 2018. – № 32 (8). – P. 4238. https://doi.org/10.1002/bmc.4238 

 

9. Detection of l-Cysteine in wheat flour by Raman microspectroscopy combined chemometrics of HCA and PCA / N. Cebi, C. E. Dogan, A. Develioglu et al. // Food Chem. – 2017. – № 228. – P. 116–124. https://doi.org/10.1016/j.foodchem.2017.01.132 

 

10. Cystine / European Pharmacopoeia 9.0. –Strasbourg: Council ofEurope, 2016. – P. 2187–2188.

 

11. Чернобровкин, М. Г. Определение аминокислот в препарате «Элтацин» / М. Г. Чернобровкин, Н. В. Кольцова, Б. Н. Шепелев // Фармация. – 2004. – № 5. – С. 18–20.

 

12. Blazheyevskiy, M. Y. Application of peroxy acids as disinfectants and sterilization agents (monograph) / M. Y. Blazheyevskiy, D. N. Riabko. – Saarbrücken (Deutschland) : LAP LAMBERT Academic Publishing, 2014.





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