Determination of L-Cystine in Tablets Using the Chemiluminescence Method




L-cystine, inhibition, luminol, chemilumeniscence, hemoglobin


Aim. To develop the method for the quantitative determination of L-cystine in sublingual tablets Elthacin® using the method of chemiluminescence inhibition in the H2L (luminol) – Н2О2 – Нb (hemoglobin) system.

Materials and methods. The study objects were the pure substance of L-cystine of pharmaceutical grade and sublingual tablets Elthacin® produced by the Medical Scientific-Production Complex “Biotics Ltd” (Russia) containing 70 mg of glycine, L-glutaminic acid and L-cystine in their composition. The intensity of chemiluminescence was measured on the device with a FEU-84-A photoelectric multiplier using an IMT-0.5 measuring instrument of low currents and a quick-acting automatic potentiometer.

Results and discussion. The method of the L-cystine quantitative determination in tablets based on the inhibition of chemiluminescence in the H2L – H2O2 – Hb system has been developed. The calibration graph was linear over the concentration range from 7 to 70 μg · mL–1. No interferences were observed in the presence of common components of the tablets, such as glycine and L-glutaminic acid. RSD = ± 2.35 %, (δ = + 1.13 %), LOD (3S) = 4 μg · mL–1, LOQ (10S) = 13 μg · mL–1 for the sublingual tablets Elthacin®.

Conclusions. The method proposed is promising for further research on the subject of its application for the determination of L-cystine in drugs.

Author Biographies

M. Ye. Blazheyevskiy, National University of Pharmacy

Doctor of Chemistry (Dr. habil), professor of the Physical and Colloid Chemistry Department

N. Yu. Bondarenko, National University of Pharmacy

Candidate of Pharmacy (Ph. D), associate professor of the
Physical and Colloid Chemistry Department

Yu. Yu. Serdiukova, National University of Pharmacy

Candidate of Pharmacy (Ph. D), teaching assistant of the Physical and Colloid Chemistry Department

V. D. Yaremenko, National University of Pharmacy

Candidate of Pharmacy (Ph. D), associate professor of the Department of Medicinal Chemistry


Blazheyevskiy, M. Y., & Moroz, V. P. (2018). Iodometric determination of cystine in pharmaceutical formulation using diperoxyadipic acid as reagent. Methods and objects of chemical analysis, 13 (1), 13–17.

Damle, M. S., Newton, L. A. A., Villalba, M. M., Leslie, R., & Davis, J. (2010). Plumbagin: A New Route to the Electroanalytical Determination of Cystine. Electroanalysis, 22 (21), 2491–2495.

Shukla, J., & Pitre, K. . (2002). Analysis of cystine in human blood for monitoring of cases of burns. Journal of Pharmaceutical and Biomedical Analysis, 27 (5), 821–826.

Shaidarova, L. G., Ziganshina, S. A., Tikhonova, L. N., & Budnikov, G. K. (2003). Electrocatalytic Oxidation and Flow-Injection Determination of Sulfur-Containing Amino Acids at Graphite Electrodes Modified with a Ruthenium Hexacyanoferrate Film. Journal of Analytical Chemistry, 58 (12), 1144–1150.

Shpak, A. V., Pirogov, A. V., Shpigun, О. A. (2003). Determination of Amino Acids by Capillary Electrophoresis without Preliminary Derivatization. Zhurnal analiticheskoi khimii, 58 (7), 317–319.

Ensafi, A. A., Rezaei, B., & Nouroozi, S. (2009). Flow injection spectrofluorimetric determination of cystine and cysteine. Journal of the Brazilian Chemical Society, 20 (2), 288–293.

Bhawani, S. A., Mohamad Ibrahim, M. N., Sulaiman, O., Hashim, R., Mohammad, A., & Hena, S. (2012). Thin-layer chromatography of amino acids: a review. Journal of Liquid Chromatography & Related Technologies, 35 (11), 1497–1516.

Jamalpoor, A., Sparidans, R. W., Pou Casellas, C., Rood, J. J. M., Joshi, M., Masereeuw, R., & Janssen, M. J. (2018). Quantification of cystine in human renal proximal tubule cells using liquid chromatography-tandem mass spectrometry. Biomedical Chromatography, 32 (8), e4238.

Cebi, N., Dogan, C. E., Develioglu, A., Yayla, M. E. A., & Sagdic, O. (2017). Detection of l-Cysteine in wheat flour by Raman microspectroscopy combined chemometrics of HCA and PCA. Food Chemistry, 228, 116–124.

10. European Pharmacopoeia 9.0. (2016). Cystine. Council of Europe:Strasbourg, 2187–2188.

Chernobrovkin, M. G., Koltcova, N. V., Shepelev, B. N. (2004). Farmatciia, 5, 18–20.

Blazheyevskiy, M. Ye., Riabko, D. N. (2014). Application of peroxy acids as disinfectants and sterilization agents (monograph). Saarbrücken (Deutschland): LAP LAMBERT Academic Publishing.






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