Determination of L-Cystine in Tablets Using the Chemiluminescence Method

Authors

DOI:

https://doi.org/10.24959/nphj.20.23

Keywords:

L-cystine, inhibition, luminol, chemilumeniscence, hemoglobin

Abstract

Aim. To develop the method for the quantitative determination of L-cystine in sublingual tablets Elthacin® using the method of chemiluminescence inhibition in the H2L (luminol) – Н2О2 – Нb (hemoglobin) system.

Materials and methods. The study objects were the pure substance of L-cystine of pharmaceutical grade and sublingual tablets Elthacin® produced by the Medical Scientific-Production Complex “Biotics Ltd” (Russia) containing 70 mg of glycine, L-glutaminic acid and L-cystine in their composition. The intensity of chemiluminescence was measured on the device with a FEU-84-A photoelectric multiplier using an IMT-0.5 measuring instrument of low currents and a quick-acting automatic potentiometer.

Results and discussion. The method of the L-cystine quantitative determination in tablets based on the inhibition of chemiluminescence in the H2L – H2O2 – Hb system has been developed. The calibration graph was linear over the concentration range from 7 to 70 μg · mL–1. No interferences were observed in the presence of common components of the tablets, such as glycine and L-glutaminic acid. RSD = ± 2.35 %, (δ = + 1.13 %), LOD (3S) = 4 μg · mL–1, LOQ (10S) = 13 μg · mL–1 for the sublingual tablets Elthacin®.

Conclusions. The method proposed is promising for further research on the subject of its application for the determination of L-cystine in drugs.

Author Biographies

M. Ye. Blazheyevskiy, National University of Pharmacy

Doctor of Chemistry (Dr. habil), professor of the Physical and Colloid Chemistry Department

N. Yu. Bondarenko, National University of Pharmacy

Candidate of Pharmacy (Ph. D), associate professor of the
Physical and Colloid Chemistry Department

Yu. Yu. Serdiukova, National University of Pharmacy

Candidate of Pharmacy (Ph. D), teaching assistant of the Physical and Colloid Chemistry Department

V. D. Yaremenko, National University of Pharmacy

Candidate of Pharmacy (Ph. D), associate professor of the Department of Medicinal Chemistry

References

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Published

2020-01-27

Issue

No. 1(99) (2020)

Section

Synthesis and Analysis of Biologically Active Substances

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