The preclinical studies of the stress-protective activity of “Tonus-aktiv” homeopathic granules

Authors

DOI:

https://doi.org/10.24959/nphj.20.36

Keywords:

homeopathy, chronic fatigue syndrome, preclinical studies, stress-protective activity

Abstract

Aim. To perform the preclinical studies of the stress-protective activity of a new “Tonus-Aktiv” homeopathic medicinal product.

Materials and methods. The stress-protective effect of the “Tonus-Aktiv” homeopathic medicinal product (MP) and the reference drug – “Stress-gran” homeopathic granules were studied on the model of neuromuscular tension by Selye. The stress-protective effect of the drugs was assessed by the conventional methods described in the scientific literature and the corresponding current instructional guidelines. All factual material was processed by variational statistics methods using parametric (Newman-Keuls test) and non-parametric methods of analysis (Kruskal-Wallis, Mann-Whitney).

Results and discussion. It was found that “Tonus-Aktiv” had a pronounced gastro-protective effect, reducing the area of ulcers by almost 3 times, having a statistically significant antioxidant effect, reducing the level of TBA (thiobarbituric acid) active products in the liver homogenate. It did not have a normalizing effect on the protein metabolism, but it did contribute to enhancing energy-supplying metabolic processes: aerobic glycolysis indicated by a reliable increase of the pyruvic acid level in the blood serum, and lipolysis, as evidenced by a significantly higher level of the total blood lipids. “Tonus-Aktiv” showed a pronounced antioxidant activity: it reduced the TBA level of active products in the liver homogenate. At the same time, the level of endogenous antioxidant of reduced glutathione was observed to be lower than in the stressed animals against the background of the “Tonus-Aktiv” application. It indicates the physiological nature of the stress protective activity of the drug.

Conclusions. A moderate stress-protective activity of “Tonus-Aktiv” homeopathic granules can be observed; it is caused by antioxidant properties, as well as a probable activating effect on the hypothalamic-pituitary-adrenal system, which may be manifested by the protective action.

Author Biographies

O. O. Gaidukova, V. N. Karazin Kharkiv National University

Candidate of Pharmacy (Ph.D.), teaching assistant of the Department of General Practice – Family Medicine

Ye. Ya. Nikolenko, V. N. Karazin Kharkiv National University

Doctor of Medicine (Dr. habil.), professor, head of the Department of General Practice – Family Medicine

G. I. Kvitchata, V. N. Karazin Kharkiv National University

Candidate of Medicine (Ph.D.), associate professor of the Department of General Practice – Family Medicine

S. V. Oliinyk, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Pharmacy (Ph.D.), teaching assistant of the Department of Technology of Drugs

L. V. Belyaeva, V. N. Karazin Kharkiv National University

Candidate of Biology (Ph.D.), associate professor of the Department of General Practice – Family Medicine

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Published

2020-09-24

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Section

Experimental and Clinical Pharmacology