The study of the anti-inflammatory effect of thick extracts of Tanacetum parthenium L. herb on the model of acute inflammation

Authors

  • O. Ya. Mishchenko Institute for Advanced Training of Pharmacy Specialists of the National University of Pharmacy of the Ministry of Health of Ukraine , Ukraine https://orcid.org/0000-0001-5862-4543
  • O. V. Andriianenkov Institute for Advanced Training of Pharmacy Specialists of the National University of Pharmacy of the Ministry of Health of Ukraine , Ukraine https://orcid.org/0009-0006-4209-756X

DOI:

https://doi.org/10.24959/nphj.26.205

Keywords:

anti-inflammatory effect; thick lipophilic extract of Tanacetum parthenium L. herb (LETP); thick hydrophilic extract of Tanacetum parthenium L. herb (HETP); zymosan-induced edema.

Abstract

Aim. To study the anti-inflammatory properties of newly created extracts of Tanacetum parthenium (L.) on the model of acute inflammation – zymosan-induced paw edema in rats.

Materials and methods. The work investigated the thick extracts of Tanacetum parthenium L.: lipophilic (chloroform) – LETP and hydrophilic (hydroalcoholic) – HETP. To study the anti-inflammatory effect of LETP and HETP, a model of paw edema in rats (subplantar injection of 2 % zymosan solution into the left hind limb) was used. Thirty (30) white non-linear sexually mature male rats with an initial weight of 160-180 g were used. The animals were divided into 5 groups: the first – control pathology (CP), the animals of which received the solvent (distilled water), the second and third – animals received intragastric HETP and LETP in the dose of 50 mg/kg. The fourth and fifth groups of animals, respectively, received intragastric reference drugs – quercetin granules in the dose of 200 mg/kg and diclofenac sodium tablets in the dose of 5 mg/kg. The anti-inflammatory effect of the drugs was assessed by inhibiting the development of the limb edema in dynamics (for 0.5, 1, 2, 3 hours) compared to animals of the control pathology group. The foot volume was determined using a plethysmometer (PanLab LE7500, Spain).

Results. HETP showed almost the same anti-inflammatory effect throughout the entire study period (30.6–39.2 %), in the first 30 min – at the level of 36.6 % (p < 0.05), during the prostaglandin phase – 39.2 % (p < 0.05). This dynamics of the anti-inflammatory effect of HETP probably indicates the ability of the biologically active substances of the extract to inhibit both the release of leukotrienes and prostaglandins. Compared with other agents studied, LETP showed the lowest anti-inflammatory effect throughout the entire study period (at the level of 14.3 %).

Conclusions. The results obtained regarding the severity of the anti-inflammatory activity of HETP determined in the dynamics on the zymosan-induced inflammation model indicate that its implementation has the inhibitory effect on the release of inflammatory mediators, in particular, leukotrienes and prostaglandins. LETP showed the least anti-inflammatory effect throughout the entire study period. Taking into account the above, HETP was chosen for further research as showing both analgesic and anti-inflammatory activity.

References

  1. Liu, C. H., Abrams, N. D., Carrick, D. M., Chander, P., Dwyer, J., Hamlet, M. R. J., Macchiarini, F., PrabhuDas, M., Shen, G. L., Tandon, P., & Vedamony, M. M. (2017). Biomarkers of chronic inflammation in disease development and prevention: Challenges and opportunities. Nature Immunology, 18, 1175–1180.
  2. Virshette, S. J., Patil, M. K., Somkuwar, A. P., & Jadhav, R. B. (2019). A review on medicinal plants used as anti-inflammatory agents. Journal of Pharmacognosy and Phytochemistry, 8, 1641–1646.
  3. Kim, Y., Bayona, P. W., Kim, M., Chang, J., Hong, S., Park, Y., Budiman, A., Kim, Y. J., Choi, C. Y., Kim, W. S., Lee J., & Cho, K. W. (2018). Macrophage lamin A/C regulates inflammation and the development of obesity-induced insulin resistance. Frontiers in Immunology, 9, 1–14.
  4. Mozos, I., Malainer, C., Horbańczuk, J., Gug, C., Stoian, D., Luca, C. T., & Atanasov, A. G. (2017). Inflammatory markers for arterial stiffness in cardiovascular diseases. Frontiers in Immunology, 8, 1058.
  5. Qi, H., Yang, S., Zhang, L., Neale, M. J., & Qu, J. (2017). Neutrophil extracellular traps and endothelial dysfunction in atherosclerosis and thrombosis. Frontiers in Immunology, 8, 928.
  6. González-Costa, M., & Padrón González, A. A. (2018). Inflammation from an immunologic perspective: A challenge to medicine in the 21st century. Revista Habanera de Ciencias Médicas, 18, 30–44.
  7. Bedard, N. A., Schoenfeld, A. J., & Kim, S. C. (2020). Optimum designs for large database research in musculoskeletal pain management. The Journal of Bone and Joint Surgery American, 102(1), 54. https://doi.org/10.2106/JBJS.20.00001
  8. Maayah, Z. H., Takahara, S., Ferdaoussi, M., Dyck, J. R. B., & El-Kadi, A. O. S. (2020). The anti-inflammatory and analgesic effects of formulated full-spectrum cannabis extract in the treatment of neuropathic pain associated with multiple sclerosis. Inflammation Research, 69(6), 549–558. https://doi.org/10.1007/s00011-020-01341-1
  9. Chibber, P., Kumar, C., Singh, A., Singh, I., & Kumar, S. (2020). Anti-inflammatory and analgesic potential of OA-DHZ: A novel semisynthetic derivative of dehydrozingerone. International Immunopharmacology, 3(83), 106469. https://doi.org/10.1016/j.intimp.2020.106469
  10. Onigbinde, A. T., M’Kumbuzi, V., Olaogun, M. O., & Akinpelu, A. O. (2014). Side effects of non-steroidal anti-inflammatory drugs: The experience of patients with musculoskeletal disorders. American Journal of Health Research, 2(4), 106–112.
  11. Harirforoosh, S., Asghar, W., & Jamali, F. (2013). Adverse effects of nonsteroidal anti-inflammatory drugs: An update of gastrointestinal, cardiovascular and renal complications. Journal of Pharmacy Pharmaceutical Sciences, 16, 821–847.
  12. Huang, J., Nguyen, V., Tang, X., Zhang, H., Li, Y., & Chen, J. (2016). Protection from diclofenac-induced liver injury by Yulangsan polysaccharide in a mouse model. Journal of Ethnopharmacology, 193, 207–213.
  13. Li, S., Xu, Y., Guo, W., Chen, F., & Li, J. (2020). The impacts of herbal medicines and natural products on regulating the hepatic lipid metabolism. Frontiers in Pharmacology, 24(11), 351. https://doi.org/10.3389/fphar.2020.00351
  14. Azab, A. N., & Nassar, A. (2016). Anti-inflammatory activity of natural products. Molecules, 21, 1321.
  15. Pareek, A., Suthar, M., Rathore, G., & Bansal, V. (2011). Feverfew (Tanacetum parthenium L.): A systematic review. Pharmacognosy Reviews, 5(9), 103–110.
  16. Di Giacomo, V., Ferrante, C., Ronci, M., Cataldi, A., Di Valerio, V., Rapino, M., & Menghini, L. (2019). Multiple pharmacological and toxicological investigations on Tanacetum parthenium and Salix alba extracts: Focus on potential application as anti-migraine agents. Food and Chemical Toxicology, 133, 110783.
  17. Hordiei, K. R., & Hontova, T. M. (2020). Standartyzatsiia hustogo lipofilnoho ekstraktu iz travy maruny divochoi. In Tekhnolohichni ta biofarmatsevtychni aspekty stvorennia likarskykh preparativ riznoi napravlenosti dii (pp. 152–153). National University of Pharmacy.
  18. Hordiei, K. R., Hontova, T. M., & Kotova, E. E. (2021). Doslidzhennia yakisnoho skladu ta kilkisnoho vmistu fenolnykh spoluk u travi maruny divochoi ta sorti Phlora pleno. In Planta+: Nauka, praktyka ta osvita (pp. 71–73). Palyvoda A. V.
  19. Derzhavne pidpryiemstvo «Derzhavnyi naukovyi tsentr likarskykh zasobiv i medychnoi produktsii». (2011). Nastanova ST-N MOZU 42-3.0:2011 «Likarski zasoby. Farmatsevtychna rozrobka (ICH Q8)». Ministerstvo okhorony zdorovia Ukrainy. https://compendium.com.ua/uk/clinical-guidelines-uk/standartizatsiya-farmatsevtichnoyi-produktsiyi-tom-1/st-n-mozu-42-3-0-2011/
  20. European convention for the protection of vertebrate animals used for the experimental and other scientific purproses. European Treaty Series № 123. https://rm.coe.int/168007a67b
  21. Drohovoz, S. M., Zupanets, I. A., Mokhort, M. A., Yakovleva, L. V., & Klebanov, B. M. (2001). Eksperymentalne (doklinichne) vyvchennia farmakolohichnykh rechovyn, yaki proponuiutsia yak nesteroidni protyzapalni zasoby. U O. V. Stefanova (Red.). Doklinichni doslidzhennia likarskykh zasobiv: metod. rek. (pp. 292–320). Avitsena.
  22. Mishchenko, O. Y., & Kyrychenko, I. V. (2022). Analgetychni vlastyvosti ekstraktiv travy pyzhmy divochoi (Tanacetum parthenium). Farmakolohiia ta likarska toksykolohiia, 16(3), 167–175. https://doi.org/10.33250/16.03.167
  23. Kvertsetyn (Quercetinum). Kompendium. https://compendium.com.ua/info/4601/kvertsetin/
  24. Ulanova, I. P., Sidorov, K. K., & Halepo, A. I. (1968). To the question of accounting for the body surface of experimental animals in toxicological research. Medicine, 10, 18–25.
  25. Bondarenko, Y. S., & Kravchenko, S. V. (2018). Posibnyk do vyvchennia dystsypliny «Statystychnyi analiz danykh». Lira.
  26. Pugh, W. J., & Sambo, K. (1988). Prostaglandin synthetase inhibitors in feverfew. Journal of Pharmacy and Pharmacology, 40, 743–745.
  27. Recinella, L., Chiavaroli, A., Di Giacomo, V., Orlando, G., Ferrante, C., & Brunetti, L. (2020). Anti-inflammatory and neuromodulatory effects induced by Tanacetum parthenium water extract: Results from in silico, in vitro and ex vivo studies. Molecules, 23, 14–22.
  28. Yuan, A., Gong, L., Luo, L., Chen, Z., & Lu, Y. (2017). Revealing anti-inflammation mechanism of water-extract and oil of Forsythiae fructus on carrageenan-induced edema rats by serum metabolomics. Biomedicine Pharmacotherapy, 95, 929–937.
  29. Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., & Bitto, A. (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 8416763. http://doi.org/10.1155/2017/8416763
  30. Miao, M., & Xiang, L. (2020). Pharmacological action and potential targets of chlorogenic acid. Advances in Pharmacology, 87, 71–88.
  31. Xu, J. G., Hu, Q. P., & Liu, Y. (2012). Antioxidant and DNA-protective activities of chlorogenic acid isomers. Journal of Agricultural and Food Chemistry, 60(46), 11625–11630.
  32. Tajner-Czopek, A., Gertchen, M., Rytel, E., & Kita, A. (2020). Study of antioxidant activity of some medicinal plants having high content of caffeic acid derivatives. Antioxidants (Basel), 9(5), 412.
  33. Enogieru, A. B., Haylett, W., Hiss, D. C., Bardien, S., & Ekpo, O. E. (2018). Rutin as a potent antioxidant: Implications for neurodegenerative disorders. Oxidative Medicine and Cellular Longevity, 27, 6241017.
  34. Li, Y., Wang, P., Xiao, W., Sun, J., & Zhang, Y. (2013). Screening and analyzing the potential bioactive components from reduning injection using macrophage cell extraction and ultra-high performance liquid chromatography coupled with mass spectrometry. American Journal of Chinese Medicine, 41(1), 221–229.
  35. Hwang, S. J., Kim, Y., Park, Y., Lee, H. J., & Kim, K. W. (2014). Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells. Inflammation Research, 63, 81–90.
  36. Yun, N., Kang, J., & Lee, S. (2012). Protective effects of chlorogenic acids against ischemia/reperfusion injury in rat liver: Molecular evidence of its antioxidant and anti-inflammatory properties. Journal of Nutritional Biochemistry, 23, 1249–1255.
  37. Gao, X. H., Zhang, S. D., Wang, L. T., Yu, L., Zhao, J., & Liu, Y. (2020). Anti-inflammatory effects of neochlorogenic acid extract from mulberry leaf (Morus alba L.) against LPS-stimulated inflammatory response through mediating the AMPK/Nrf2 signaling pathway in A549 cells. Molecules, 25(6), 1385.
  38. Mishchenko, O., Kyrychenko, I., Gontova, T., Kalko, K., & Hordiei, K. (2022). Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract. ScienceRise: Pharmaceutical Science, 39(5), 91–99. https://doi.org/10.15587/2519-4852.2022.266400

Downloads

Published

2026-03-31

Issue

Vol. 111 No. 1 (2026): News of Pharmacy

Section

Articles

License

Copyright (c) 2026 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.