The parameters optimization of liquefied gas extraction of the yellow bedstraw overground part by the response surface methodology
DOI:
https://doi.org/10.24959/nphj.14.1972Keywords:
yellow bedstraw (Galium verum L.), extraction, difluorochloromethane, response surface methodology, Box-Behnken designAbstract
For the first time quantitative effects of liquefied gas extraction temperature, the correlation of the extractant volume and the raw material mass, humidity and the average size of the raw material particles have been analyzed in the lipophilic complex (LC) yield from the overground part of yellow bedstraw (Galium verum L.) using the response surface methodology (RSM). For this research, the four-factor and the three-level Box-Behnken design has been chosen; it contains four covariates, namely the extraction temperature – X1, the ratio of the extractant volume (ml) and the raw material mass (g) – X2, the raw material humidity – X3 and the average size of the raw material particles – X4 based on the results of one-factor test. Twenty seven experimental studies have been conducted with three repeats in the centre of the design to assess the proper operational margin of the sum of squares. The experimental results obtained have been set at the polynomial equations of the second order using the multiple regression analysis, and analyzed by the corresponding statistical methods. With the help of solving the regression equation and by means of the analysis of the response surface of contour plots the optimal conditions of the LC extraction have been determined: the extraction temperature – 35°C, the ratio of the extractant volume (ml) and the raw material mass (g) – 8, the raw material humidity – 7% and the average size of the raw material particles – 1 mm. It has been found that the most significant factors for the process under research are the extraction temperature, the ratio of the extractant volume (ml) and the raw material mass (g), and the raw material humidity. Comparison of the data obtained with the data of literature sources confirms the relevance of the subcritical difluorochloromethane choice as an extractant for the LC obtaining. Summarizing all above-mentioned information it may be concluded that the use of liquefied difluorochloromethane for the LC extraction from the overground part of yellow bedstraw (Galium verum L.) is promising from the viewpoint of the modern pharmaceutical technology, and the use of the RSM as an instrument for the process optimization allows reducing the costs for carrying out the pharmaceutical development.
References
Гарна С.В., Вєтров П.П. // Запорож. мед. журн. – 2010. – №3. – С. 92-94.
Зилфикаров И.Н. Обработка лекарственного растительного сырья сжиженными газами и сверхкритическими флюидами. – Пятигорск, 2007. – 136 с.
ІльїнаТ.В. // УБФЖ. – 2009. – №5. – С. 31-36.
Пат. 105231 Україна МПК (2006) A 61 K 36/74, A 61 K 35/00. – Опубл.: 25.04.14. – Бюл. 8.
Annadurai G. // J. Hazard. Mater. – 2008. – Vol. 151. – Р. 171-178.
Bae S. Shoda M. // Biotechnol. Bioeng. – 2005. – Vol. 90, №1. – Р. 20-28.
Chun-Chao Z., Jian-Hua S., Xian L. et al. // Nat. Prod. Res. – 2006. – Vol. 20, №11. – Р. 981-984.
Demirezer L.Ö., Gürbüz F. // Turk. J. Chem. – 2006. – Vol. 30. – Р. 525-534.
Ferreira S.L.C., Bruns R.E. // Anal. Chim. Acta. – 2007. – Vol. 597. – Р. 179-186.
Li Q.J., Nan M.L. // J. Med. Plant. – 2010. – Vol. 1, №10. – Р. 5-6, 9.
Lu J., Zhou C. // Adv. J. of Food Sci. Technol. – 2013. – Vol. 5(3). – Р. 310-317.
Milić P.S. // CI&CEQ. – 2013. – Vol. 19(1). – Р. 141-152.
Milić P.S., Rajkovic K.M. // Ultrason. Sonochem. – 2013. – Vol. 20. – Р. 525-534.
Moghaddam M.G., Khajeh M. // Food and Nutrition Sci. – 2011. – Vol. 2. – Р. 803-808.
Proskochilo A.V., Demianenko V.G. // J. Chem. Pharm. Res. – 2014. – Vol. 6 (2). – Р. 765-771.
Shaibu A.B., Cho B.R. // Int. J. Adv. Manuf. Technol. – 2009. – Vol. 41. – Р. 631-641.
Wang J., Zhang J. // Carbohydr. Polym. – 2010. – Vol. 80. – Р. 84-93.
Xu L., Liu J. // Sep. Sci. Technol. – 2013. – Vol. 48. – Р. 2779-2785.
Yang L., Jiang J.G. // J. Sep. Sci. – 2009. – Vol. 32. – Р. 1437-1444.
Yin G., Dang Y. // Carbohydr. Polym. – 2008. – Vol. 74. – Р. 603-610.
Zhao C., Shao J. // China J. of Chinese Materia Medica. – 2009. – Vol. 34, №21. – Р. 2761-2763.
Downloads
Published
Issue
Section
License
Copyright (c) 2014 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).