DOI: https://doi.org/10.24959/nphj.15.2002

The antimicrobial activity of silver nanoparticles in vitro

A. O. Pryskoka, A. V. Rudenko, L. S. Reznichenko, T. G. Gruzina, Z. R. Ulberg, I. S. Chekman

Abstract


Silver nanoparticles possess a high potential as an antimicrobial substance against a wide spectrum of bacteria, including antibiotic-resistant strains. Antimicrobial properties of silver nanoparticles with 30 nm in diameter synthesized according to the original protocol have been determined in this study. In in vitro study using the serial dilutions method in the solid medium the minimal inhibition concentration (MIC) of silver nanoparticles against such test-strains as Staphylococcus aureus MRSA ATCC 43300, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 2592, Shigella sonnei, Salmonella typhimurium 144 was equal to 33.46 μg/ml. MIC against B. subtilis ATCC 6633 was 133.8 μg/ml. The antimicrobial activity of silver nanoparticles has been studied on clinical isolates with multiple drug resistance isolated from wounds, urine, endocervical and faucial scrapings in surgical patients with Klebsiella ozaenae 4348, Citrobacter freundii 4369, Escherichia coli 4358, Enterobacter aerogenes 2476, Proteus mirabilis 4363, Staphylococcus aureus 4312 and Pseudomonas aeruginosa 283. The total inhibition of the microorganisms growth under the action of both doses of silver nanoparticles studied – 10 μg and 20 μg has been observed.

Keywords


silver nanoparticles; antimicrobial activity; spectrum of bacteria

Full Text:

PDF

References


Грузіна Т.Г., Дибкова С.М., Прискока А.О та ін. // Фармакологія та лікарська токсикологія. – 2012. – №3. – С. 40 – 46.

Методические указания МУК 4.2.1890-04 // Клин. микробиол. антимикроб. химиотер. – 2004. – Т.6, №4. – С. 306-359.

Патон Б.Є., МоскаленкоВ.Ф., Чекман І.С., Мовчан Б.О. // Вісник Національної академії наук України. – 2009. – № 6. – С. 76–80.

Чекман І.С. Нанофармакологія. – К.: Задруга, 2011. – 424 с.

Чекман І. С., Симонов П.В. Природні наноструктури та нано-механізми. - К.: Задруга, 2012. – 104 с.

Чекман І.С., Ульберг З.Р., Маланчук В.О. та ін. Нанонаука, нанобіологія, нанофармація. – К.: Поліграф плюс, 2012. – 328 с.

Banerjee M., Mallick S., Paul A. et al. // Langmuir. – 2010. – Vol. 26, №8. – P. 5901 – 5908.

Bhattacharaya R., Mukherjee P. //Advanced drug delivery reviews. – 2008. – Vol. 60. – P. 1289 – 1306.

Bhol K.C., Alroy J., Schechter P. J. //Clin. Exp. Dermatol. – 2004. – Vol. 26, №3. – P. 282 – 287.

Das V.L., Tomas R., Varghese R.T. et al // 3 Biotech. – 2014. – Vol. 4. – P. 121 – 126.

Dunn K.// Burns. – 2004. – Vol. 30. – P. 1 – 9.

Gunawan C., Teoh W.Y., Marquis C.P. et al. // Small. – 2013. – Vol. 9, №21. – P. 3554–3560.

Hong H.A., Khaneja R.; Tam N. et al. // Research in Microbiology. – 2009. – Vol. 160, № 2. – P. 134 – 143.

Jain J., Arora S., Rajwade J. M. et al. // Mol. Pharm. – 2009. – Vol. 6, №5. – P. 1388 – 1401.

Kahru A., Dubourguier H.-C. // Toxicology. – 2010. – Vol. 269. – P. 105 – 119.

Kalimuthu K., Babu R.S., Venkataraman D. et al. // Colloids and Surfaces B: Biointerfaces. – 2008. – Vol. 65. – P. 150 – 153.

Li Q., Mahendra D., Lyon D. et al. // Water research. – 2008. – Vol. 42. – P. 4591 – 4602.

Nagy A., Harrison A., Sabbani S. et al. // Int. J. Nanomedicine. – 2011. – Vol. 6. – P. 1833 – 1852.

Pal. S., Tak K. Y., Song J. M. // Applied and environmental Microbiology. – 2007. – Vol. 73, № 6. – P. 1712 – 1720.

Roe D., Karandikar B., Bonn-Savage N. et al. // Journal of antimicrobial chemotherapy. – 2008. – Vol. 61. – P. 869 – 876.

Zhang W., Li Y., Niu J., Chen Y. // Langmuir. – 2013. – Vol. 29, №15. – P. 4647 – 4651.


GOST Style Citations






Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Vìsn. farm.

ISSN 2415-8844 (Online), ISSN 1562-7241 (Print)