The study of amino acid composition of sapropel by the capillary electrophoresis method


  • O. Ye. Strus Lviv National Medical University named after Danylo Halytsky, Ukraine



sapropel, capillary electrophoresis method, amino acid composition


A diverse composition of sapropels allows to use them successfully in cosmetology. Sapropelic therapeutic muds exhibit the anti-inflammatory and desensitizing effect, protect the body from the damaging effects of free radicals, slow down the aging processes, give the skin freshness and elasticity, improve the cell regeneration by 10%, moisturize and increase elasticity and thickness of the keratoid layer of the epidermis. A wide range of indications for using sapropels demonstrates the importance of their application for prevention and treatment of diseases, and the relevance and prospects for their further studies to obtain new pharmaceuticals and cosmetics. When conducting research by the capillary electrophoresis method the “Kapel’ – 105/105 M” system with a positive polarity of a high voltage source (the internal diameter of the capillary is 50 μm, the full length of the capillary is 75 cm, the effective length is 65 cm) equipped with a special software for personal computers was applied. The amino acid composition of sapropel from Prybych deposits (Shatsky district, Volyn region) has been studied. The presence of a number of amino acids in sapropel with the total content of more than 2% has been found using capillary electrophoresis. Sapropel contains a vast amount of essential amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine) and a number of nonessential ones (alanine, arginine, glycine, proline and serine). Alanine, glycine, leucine, and isoleucine prevail in the mixture analyzed.


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