The spectrophotometric study of thioctic acid solutions to develop the method of its quantitative determination
Type II diabetes mellitus is a heterogeneous and multifactorial disease, which develops in the metabolic syndrome and significantly increases the risk of developing complications, such as angiopathy, neuropathy, retinopathy, nephropathy. Therefore, creation of new drugs that will affect all links of pathogenetic mechanism of the disease is presently topical. The main line treatment of the abovementioned pathologies is the use of antioxidant therapy. Antioxidants are important on different “lines of defense”: in the extracellular fluid, membrane and the cell cytosol. Thioctic acid belongs to these substances. The analysis of thioctic acid is of some difficulties as it has high propensity for different transformations, first of all, oxidation and polymerization. Therefore, the search of cost-neutral and at the same time rapid and effective methods of drug analysis remains relevant.
Aim. To study the possibility to determine thioctic acid by the spectrophotometric method.
Materials and methods. The object of the study was the substance of thioctic acid. Adsorption spectra of the solutions obtained was recorded on an Evolution 60-S spectrophotometer in the cells with the layer thickness of 10 mm.
Results and discussion. It has been found that the adsorption spectrum of thioctic acid solution in 0.1 M hydrochloric acid solution contains a wide absorption band with the maximum at 334 nm; it can be used to quantify the concentration of thioctic acid solutions by the method of one-component one-wave spectrophotometry according to the method of standard.
Conclusions. The study conducted shows the possibility to quantify the concentration of thioctic acid solutions in solutions of hydrochloric acid that model the acidic medium of the gastric juice by the method of one-component onewave spectrophotometry according to the method of standard in the maximum at 334 nm.
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Bilska A, Wtodek, L. (2005). Lipoic acid – the drug of the future? Pharmacological Reports, 57, 570–577.
Rajkumar, B., Bhavya, T., Ashok Kumar, A. (2014). Reverse Phase HPLC Method Development and Validation for the Simultaneous
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and α–Lipoic Acid in Combination Tablet. Research and Reviews: Journal of Pharmaceutical Analysis, 3 (3), 28–36.
GOST Style Citations
1. Bajaj, S. Antioxidants and diabetes / S. Bajaj, A. Khan // Indian J. of Endocrinol. and Metabolism. – 2012. – Vol. 16. – P. 267–271.
2. Bilska, A. Lipoic acid – the drug of the future? / A. Bilska, L. Wtodek // Pharmacol. Reports. – 2005. – Vol. 57. – P. 570–577.
3. Rajkumar, B. Reverse Phase HPLC Method Development and Validation for the Simultaneous Quantitative Estimation of Alpha Lipoic
acid and Allopurinol in tablets / B. Rajkumar, T. Bhavya, A. Ashok Kumar // Intern. J. of Pharmacy and Pharmac. Sci. – 2014. – Vol. 6,
Issue 1. – P. 307–312.
4. Evans, J. L. A–Lipoic Acid : A Multifunctional Antioxidant That Improves Insulin Sensitivity in Patients with Type 2 Diabetes / J. L. Evans,
I. D. Goldfine // Diabetes Technol. & Therapeutics. – 2000. – Vol. 2, Issue 3. – P. 401–413. doi: 10.1089/15209150050194279
5. Patel, D. J. Development and Validation of Spectroscopic Method for Simultaneous Estimation of Allopurinol and α–Lipoic Acid in
Combination Tablet / D. J. Patel, V. C. Jain, H. A. Raj // Res. and Reviews : J. of Pharmac. Analysis. – 2014. – Vol. 3, Issue 3. – P. 28–36.
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