Experimental study of the cream with cerium dioxide nanoparticles on the model of photodynamic injury in guinea pigs
Aim. To study the photoprotective action of the cream with cerium dioxide nanoparticles (CDN) on the model of the photodynamic injury in guinea pigs.
Materials and methods. CDN were synthesized in OOO “NanoMedTech”, the cream with CDN was developed in SSI “Institute for Single Crystals” of the NAS of Ukraine. The photodynamic injury in guinea pigs was modeled with an UV-emitter. The cream with CDN was applied preventively in the dose of of 2 mg/cm2. The erythema intensity was assessed according to S. V. Suvorov colorimetric scale, then the photoprotective activity (PPA) was calculated. The skin temperature was measured within 4 hours after exposure. The wound healing action was assessed as the number of days till complete healing of the skin of guinea pigs.
Results and discussion. The photoprotective action (PPA – 43.2 %) of the cream with CDN exceeded that of the reference drug (the cream with titanium dioxide) since the number of ulcers and deep lesions of the skin was lower. The preventive application of the cream with CDN led to the skin temperature normalization, which confirmed the ability of CDN to prevent inflammation. The wound healing action of the cream was also observed – the complete epithelization of the damaged zone took place in 5.86 days compared to 11.00 days in untreated animals.
Conclusions. The results regarding the photoprotective, wound healing action of the cream with CDN and its ability to prevent inflammation create opportunities for further study of this formulation as a photoprotector.
Maresca, V., Flori, E., Picardo, M. (2015). Skin phototype: a new perspective. Pigment Cell & Melanoma Research, 28 (4), 378–389. https://doi.org/10.1111/pcmr.12365
Didona, D., Paolino, G., Bottoni, U., Cantisani, C. (2018). Non melanoma skin cancer pathogenesis overview. Biomedicines, 6 (1), 6. https://doi.org/10.3390/biomedicines6010006
Martens, M. C., Seebode, C., Lehmann, J., Emmert, S. (2018). Photocarcinogenesis and skin cancer prevention strategies: an update. Anticancer Research, 38 (2), 1153–1158. https://doi.org/10.21873/anticanres.12334
Sample, A., He, Y. Y. (2018). Mechanisms and prevention of UV-induced melanoma. Photodermatology, Photoimmunology & Photomedicine, 34 (1), 13–24. https://doi.org/10.1111/phpp.12329
Lippens, S., Hoste, E., Vandenabeele, P., Agostinis, P., Declercq, W. (2009). Cell death in the skin. Apoptosis : an International Journal on Programmed Cell Death, 14 (4), 549–569. https://doi.org/10.1007/s10495-009-0324-z
Dunaway, S., Odin, R., Zhou, L., Ji, L., Zhang, Y., Kadekaro, A. L. (2018). Natural antioxidants: multiple mechanisms to protect skin from solar radiation. Frontiers in Pharmacology, 9, 392. https://doi.org/10.3389/fphar.2018.00392
Gulson, B., McCall, M. J., Bowman, D. M., Pinheiro, T. (2015). A review of critical factors for assessing the dermal absorption of metal oxide nanoparticles from sunscreens applied to humans, and a research strategy to address current deficiencies. Archives of Toxicology, 89 (11), 1909–1930. https://doi.org/10.1007/s00204-015-1564-z
Gilbert, E., Pirot, F., Bertholle, V., Roussel, L., Falson, F., Padois, K. (2013). Commonly used UV filter toxicity on biological functions: review of last decade studies. International Journal of Cosmetic Science, 35 (3), 208–219. https://doi.org/10.1111/ics.12030
Shukla, R. K., Sharma, V., Pandey, A. K., Singh,S., Sultana, S., Dhawan, A. (2011). ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicology in vitro : an International Journal Published in Association with BIBRA, 25 (1), 231–241. https://doi.org/10.1016/j.tiv.2010.11.008
Yefanov, V. S., Zaychenko, G. V., Nikitina, N. S., Pokotylo, O. A. (2017). Doslidzhennia hostroi toksychnosti kremu z nanochastynkamy dioksydu tseriu. V natsionalnyi zizd farmakolohiv Ukrainy. Abstract Book, 43.
Pokotylo, O. A., Nikitina, N. S., Zaychenko, G. V. (2018). The study of subchronic toxicity of the cream with cerium dioxide nanoparticles. Topical Issues of New Drugs Development. Abstract Book, 335–336.
Li, Y., Li, P., Yu, H., Bian, Y. (2016). Recent advances (2010–2015) in studies of cerium oxide nanoparticles’ health effects. Environmental Toxicology and Pharmacology, 44, 25–29. https://doi.org/10.1016/j.etap.2016.04.004
Stefanov, A. V. (1998). Bioskrining. Lekarstvennye sredstva. Kyiv: Avitsenna, 189.
Suvorov, S. V., Rabkin, E. B., Chernyshova, V. I. (1977). Quantitative evaluation of erythema of the skin. Bulletin of Experimental Biology and Medicine, 83, 284. https://doi.org/10.1007/BF00799448
Zupanets, I. A. (2005). Klinicheskaia laboratornaia diagnostika: metody issledovania. Kharkiv: Zolotye stranitsy, 200.
Bosch, R., Philips, N., Suarez-Perez, J. A., Juarranz, A., Devmurari, A., Chalensouk-Khaosaat, J., Gonzalez, S. (2015). Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals. Antioxidants (Basel, Switzerland), 4 (2), 248–268. https://doi.org/10.3390/antiox4020248
Pasparakis, M., Haase, I., Nestle, F. O. (2014). Mechanisms regulating skin immunity and inflammation. Nature Reviews. Immunology, 14 (5), 289–301. https://doi.org/10.1038/nri3646
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