Saponins of the extracts of Galium aparine and Galium verum




cleavers, lady’s bedstraw, liquid extract, saponins, HPLC


Aim. To determine the qualitative composition and the quantitative content of saponins in the liquid extracts of the herb of cleavers (Galium aparine L.) and lady’s bedstraw (Galium verum L.).

Materials and methods. Extracts of the herb of cleavers and lady’s bedstraw were obtained by the triple extraction of the raw material with 96 % ethanol when heating with the subsequent concentration of the combined extracts to the ratio of the raw material : the finished product of 1:1. Saponins were studied by the method of high-performance liquid chromatography (HPLC).

Results. As a result of the chromatographic study of extracts of the herb of cleavers and lady’s bedstraw it has been found that they contain 6 and 7 saponins, respectively. The compounds belong to ursane (ursolic, euscaphic, tormentic acids and uvaol), oleanane (oleanolic acid) and lupane (betulin and lupeol) type. In the extract from the herb of cleavers the compounds of ursane derivatives (50 mg/ml) prevail, the dominant compound is euscaphic acid (3.34 mg/ml); in the extract from the herb of lady’s bedstraw there are saponins of lupane derivatives (2.50 mg/ml), lupeol (1.60 mg/ml) is predominant.

Conclusions. The results obtained indicate the prospects for further in-depth study of the chemical composition and biological activity of the liquid extracts from the herb of cleavers and lady’s bedstraw.

Author Biographies

I. L. Shynkovenko, National University of Pharnacy

postgraduate student of the Pharmacognosy Department

T. V. Ilyina, National University of Pharnacy

Doctor of Pharmaceutical Sciences, Associate Professor. Department of Pharmacognosy

A. M. Kovalyova, National University of Pharnacy

Doctor of Pharmacy (Dr. habil.), professor of the Pharmacognosy Department

O. V. Goryacha, National University of Pharnacy

Candidate of Pharmacy (Ph.D.), teaching assistant of the Pharmacognosy Department

O. I. Golembiovska, Institute of Organic Chemistry of NAS of Ukraine

Candidate of Pharmacy (Ph.D.), engineer of the Organic Sulfur Chemistry Department, Laboratory of Condensed Heterocyclic Systems

O. M. Koshovyi, National University of Pharnacy

Doctor of Pharmacy (Dr. habil.), head of the Pharmacognosy Department


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Synthesis and Analysis of Biologically Active Substances